Pohlman R F, Fedoroff N V, Messing J
Cell. 1984 Jun;37(2):635-43. doi: 10.1016/0092-8674(84)90395-7.
We have determined the nucleotide sequence of the transposable maize controlling element Activator (Ac). The Ac element is 4563 bp long and has an imperfect terminal repetition of 11 bp. The element contains two open reading frames (ORF) encoding polypeptides of 839 and 210 amino acids. Evidence derived from structural analysis of a closely related, but transposition-defective Dissociation (Ds) element indicates that the large ORF is the structural gene for a trans-acting function required for transposition. The two ORFs diverge from a short intergenic region which contains characteristic eucaryotic transcription initiation sequences.
我们已经确定了玉米转座控制元件激活子（Ac）的核苷酸序列。Ac元件长4563碱基对，具有11碱基对的不完全末端重复序列。该元件包含两个开放阅读框（ORF），分别编码839个和210个氨基酸的多肽。对一个密切相关但转座缺陷的解离（Ds）元件进行结构分析得到的证据表明，大的ORF是转座所需反式作用功能的结构基因。这两个ORF从一个包含典型真核转录起始序列的短基因间隔区开始分歧。
