Fedoroff N, Wessler S, Shure M
Cell. 1983 Nov;35(1):235-42. doi: 10.1016/0092-8674(83)90226-x.
Restriction endonuclease fragments containing part of the Waxy (Wx) locus have been cloned from strains with insertion mutations at the locus caused by the controlling elements Activator (Ac) and Dissociation (Ds). Evidence is presented that the genetically defined Ac element corresponds to a 4.3 kb insertion, while the two Ds elements correspond to 4.1 kb and 2.0 kb insertions, all near the 3' end of the Wx transcription unit. The 4.1 kb Ds is almost completely homologous to the Ac element, differing by a central deletion of less than 0.2 kb. The 2.0 kb Ds element is homologous to the ends of the Ac element. Sequences homologous to the ends of the Ac element are present in many copies in the genomes examined, while there are ten or fewer copies of a sequence with homology to the center of the cloned Ac element. The Ac element at the Wx locus can be distinguished structurally from the other Ac-like sequences in the genome.
已从蜡质（Wx）位点发生由控制元件激活子（Ac）和解离子（Ds）引起的插入突变的菌株中克隆出含有该位点部分区域的限制性内切酶片段。有证据表明，遗传学上定义的Ac元件对应于一个4.3 kb的插入片段，而两个Ds元件分别对应于4.1 kb和2.0 kb的插入片段，所有这些插入片段都靠近Wx转录单元的3'端。4.1 kb的Ds与Ac元件几乎完全同源，仅在中央有一个小于0.2 kb的缺失。2.0 kb的Ds元件与Ac元件的末端同源。在所检测的基因组中，与Ac元件末端同源的序列有许多拷贝，而与克隆的Ac元件中心具有同源性的序列拷贝数为十个或更少。Wx位点的Ac元件在结构上可与基因组中其他类Ac序列区分开来。
