Zdravkovic M, Aboagye-Mathiesen G, Zachar V, Tóth F D, Dalsgård A M, Hager H, Ebbesen P
Danish Cancer Society, Department of Virus and Cancer, Aarhus.
Placenta. 1994 Sep;15(6):591-600. doi: 10.1016/s0143-4004(05)80406-0.
Human placental trophoblast cells produce predominantly interferon-beta-type (IFN-beta) when stimulated with viral inducers. The aim of the present study was to determine the in vitro antiproliferative effect of the trophoblast interferon-beta (tro-IFN-beta) on mitogen-stimulated and resting lymphocytes. The antiproliferative effect of the tro-IFN-beta was compared to human recombinant IFN-beta. All activities of tro-IFN-beta and human recombinant IFN-beta ranging between 10-1000 IU/ml showed suppression of proliferative responses on mitogen-stimulated and resting lymphocytes compared to cultures without IFN treatment. The inhibitory level of both tro-IFN-beta and recombinant IFN-beta was significantly higher on the stimulated than on the resting lymphocytes. Although there was a variation in the inhibition of lymphocyte proliferation by both IFNs with respect to time, there was no statistically significant difference in the antiproliferative effect of the IFNs on both resting and mitogen-stimulated lymphocytes. Since IFNs are produced locally by the placenta during pregnancy, our data suggest that in addition to the antiviral activity, the human tro-IFN-beta may participate in the local control of the maternal immune response during pregnancy at the fetomaternal interface.
人胎盘滋养层细胞在受到病毒诱导剂刺激时主要产生β型干扰素（IFN-β）。本研究的目的是确定滋养层β型干扰素（tro-IFN-β）对有丝分裂原刺激的淋巴细胞和静息淋巴细胞的体外抗增殖作用。将tro-IFN-β的抗增殖作用与重组人IFN-β进行比较。与未用IFN处理的培养物相比，tro-IFN-β和重组人IFN-β在10 - 1000 IU/ml范围内的所有活性均显示对有丝分裂原刺激的淋巴细胞和静息淋巴细胞的增殖反应有抑制作用。tro-IFN-β和重组IFN-β对刺激淋巴细胞的抑制水平显著高于对静息淋巴细胞的抑制水平。尽管两种IFN对淋巴细胞增殖的抑制作用随时间存在差异，但两种IFN对静息和有丝分裂原刺激的淋巴细胞的抗增殖作用在统计学上无显著差异。由于孕期胎盘在局部产生IFN，我们的数据表明，除了抗病毒活性外，人tro-IFN-β可能在妊娠期间参与母胎界面处母体免疫反应的局部调控。
