Lacoste L, Lacaille M, Brakier-Gingras L
Biochim Biophys Acta. 1976 Aug 2;442(1):88-97. doi: 10.1016/0005-2787(76)90179-9.
Mutagenesis of Escherichia coli K12 cells with ethyl methanesulfonate and selection of streptomycin-resistant mutants after a long delay for phenotypic expression allowed us to isolate new types of streptomycin-resistant ribosomes. Misreading patterns of the ribosomes in the presence of streptomycin revealed that most of the streptomycin-resistant mutants isolated under these conditions differed from the four classical types of streptomycin-resistant mutants studied and characterized by Strigini, P. and Gorini, L. (1970) J. Mol. Biol. 47, 517-530.
用甲磺酸乙酯对大肠杆菌K12细胞进行诱变，并在长时间延迟以实现表型表达后筛选出链霉素抗性突变体，这使我们能够分离出新型的链霉素抗性核糖体。在链霉素存在的情况下核糖体的错读模式表明，在这些条件下分离出的大多数链霉素抗性突变体与Strigini, P.和Gorini, L.（1970年，《分子生物学杂志》47卷，517 - 530页）所研究和表征的四种经典类型的链霉素抗性突变体不同。
