Wapnir R A, Devas G
Department of Pediatrics, North Shore University Hospital--Cornell University Medical College, Manhasset, NY 11030.
Am J Clin Nutr. 1995 Jan;61(1):105-10. doi: 10.1093/ajcn/61.1.105.
The purpose of this study was to investigate whether a high fat intake would potentiate an excess of fructose in the diet of rats to alter energy metabolism and worsen the nutritional status for copper. Weanling male rats were fed diets with 45% fat and 20% cornstarch, 45% fat and 20% fructose, 5% fat and 60% cornstarch, or 5% fat and 60% fructose for 3 wk, with either sufficient (+) or deficient (-) amounts of copper. Copper deficiency, as demonstrated by low plasma copper and ceruloplasmin, caused a decrease of liver, heart, and testes copper; a decline of liver and heart zinc; and an increase of hepatic iron. High-fat diets reduced liver glucose-6-phosphate dehydrogenase (G6PDH) and, to a lesser extent, glycerophosphate dehydrogenase (GPDH). The interaction between high-fat and high-fructose diets, superimposed on copper deficiency, resulted in a lowering of G6PDH, GPDH, and malic enzyme. These results support the hypothesis that a high fat ingestion becomes an aggravating stress factor, which, in combination with a high-fructose intake in a copper-deficient diet, adversely alters key organ mineral content, with detrimental effects on copper nutritional status and intermediary metabolism.
本研究的目的是调查高脂肪摄入是否会增强大鼠饮食中过量果糖对能量代谢的影响，并使铜的营养状况恶化。将断乳雄性大鼠分别喂食含45%脂肪和20%玉米淀粉、45%脂肪和20%果糖、5%脂肪和60%玉米淀粉或5%脂肪和60%果糖的饲料，持续3周，同时给予充足（+）或缺乏（-）量的铜。血浆铜和铜蓝蛋白水平降低表明铜缺乏，导致肝脏、心脏和睾丸铜含量下降；肝脏和心脏锌含量降低；肝脏铁含量增加。高脂肪饮食降低了肝脏葡萄糖-6-磷酸脱氢酶（G6PDH），并在较小程度上降低了甘油磷酸脱氢酶（GPDH）。高脂肪和高果糖饮食之间的相互作用，叠加在铜缺乏的基础上，导致G6PDH、GPDH和苹果酸酶水平降低。这些结果支持了这样一种假设，即高脂肪摄入成为一个加重的应激因素，与铜缺乏饮食中的高果糖摄入相结合，会对关键器官矿物质含量产生不利影响，对铜的营养状况和中间代谢产生有害作用。
