基于核糖体DNA间隔区限制位点变异的智人与相关物种的系统发育关系。

Phylogenetic relationships among Homo sapiens and related species based on restriction site variations in rDNA spacers.

作者信息

Suzuki H, Kawamoto Y, Takenaka O, Munechika I, Hori H, Sakurai S

机构信息

Division of Molecular Genetics, Jikei University School of Medicine, Tokyo, Japan.

出版信息

Biochem Genet. 1994 Aug;32(7-8):257-69. doi: 10.1007/BF00555829.

DOI:10.1007/BF00555829

PMID:7826313

Abstract

A rapid method, using 12 restriction enzymes, was employed to analyze variations in ribosomal DNA (rDNA) spacers in a study of phylogenetic relationships between Homo sapiens and related species. We mapped restriction sites in the external and internal spacer regions and compared the arrangements of sites. The estimated sequence divergence between Homo sapiens and Pan troglodytes, Pan paniscus, Gorilla gorilla, Pongo pygmaeus, Hylobates lar, H. agilis, and Macaca fuscata was 2.7, 2.3, 3.8, 7.3, 6.8, 7.8, and 14.1%, respectively. The genetic relationships inferred from these distances generally correspond to those inferred from analyses of other molecular markers in the literature. The divergence between H. lar and H. agilis and between H. lar and H. syndactylus was 0.34 and 2.4%, respectively.

摘要

在一项关于智人与相关物种系统发育关系的研究中，采用了一种使用12种限制酶的快速方法来分析核糖体DNA（rDNA）间隔区的变异。我们绘制了外部和内部间隔区的限制酶切位点图谱，并比较了位点的排列情况。智人与黑猩猩、倭黑猩猩、大猩猩、猩猩、白掌长臂猿、敏捷长臂猿和猕猴的估计序列差异分别为2.7%、2.3%、3.8%、7.3%、6.8%、7.8%和14.1%。从这些距离推断出的遗传关系总体上与文献中从其他分子标记分析推断出的关系一致。白掌长臂猿与敏捷长臂猿之间以及白掌长臂猿与合趾猿之间的差异分别为0.34%和2.4%。