Roby P, Page M
Department of Biochemistry, Faculty of Medicine, Université Laval, Québec, Canada.
Biochem Biophys Res Commun. 1995 Jan 17;206(2):792-8. doi: 10.1006/bbrc.1995.1112.
The FITC-labeled C-terminal alpha-helix portion of human MGSA/gro alpha (MGSA 47-71) was shown, using flow cytometry, to bind to human melanoma cells Hs294t but not to non-melanoma cells. MGSA (47-71) is capable to induce specifically DNA synthesis of melanoma cells. These results indicate that the C-terminal portion of human MGSA is sufficient for its biological activity, thus raising questions about the importance of dimerization and of the N-terminal ELR box of the molecule which has been proposed.
使用流式细胞术显示，人MGSA/groα（MGSA 47-71）的异硫氰酸荧光素（FITC）标记的C末端α螺旋部分可与人黑色素瘤细胞Hs294t结合，但不与非黑色素瘤细胞结合。MGSA（47-71）能够特异性诱导黑色素瘤细胞的DNA合成。这些结果表明，人MGSA的C末端部分足以发挥其生物活性，从而对已提出的该分子二聚化和N末端ELR框的重要性提出了疑问。
