Waid D K, McLoon S C
Department of Cell Biology and Neuroanatomy University of Minnesota, Minneapolis 55455.
Neuron. 1995 Jan;14(1):117-24. doi: 10.1016/0896-6273(95)90245-7.
The aim of this study is to gain insight into the time during the life history of a retinal neuron that it becomes committed to a particular phenotype. At this point, it is not possible to identify the time of commitment, but the time that differentiation begins can be identified. Bromodeoxyuridine labeling coupled with immunohistochemistry with a ganglion cell-specific antibody was used to fix the time of the beginning of ganglion cell differentiation relative to the time of mitosis in the developing chick retina. It was found that ganglion cells can begin to differentiate in less than 15 min after the end of mitosis. This suggests that the retinal ganglion cell fate may be determined before or during mitosis.
本研究的目的是深入了解视网膜神经元在其生命历程中确定为特定表型的时间。目前，尚无法确定确定的时间，但可以确定分化开始的时间。使用溴脱氧尿苷标记结合针对神经节细胞特异性抗体的免疫组织化学方法，相对于发育中的鸡视网膜有丝分裂时间，来确定神经节细胞分化开始的时间。结果发现，神经节细胞在有丝分裂结束后不到15分钟就可以开始分化。这表明视网膜神经节细胞的命运可能在有丝分裂之前或期间就已确定。
