Jackson M, Choo L P, Watson P H, Halliday W C, Mantsch H H
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Manitoba.
Biochim Biophys Acta. 1995 Jan 25;1270(1):1-6. doi: 10.1016/0925-4439(94)00056-v.
Infrared spectra of human central nervous system tissue and human breast carcinoma are presented. The spectra are discussed in terms of the composition of the tissues. It is shown that differences between spectra of white and grey matter can be rationalised on the basis of differences in lipid content. Spectra of the choroid plexus and arachnoid villus of the meninges show a series of absorptions not observed in other CNS tissue. These absorptions are discussed in terms of the connective tissue content of the samples. We demonstrate that the presence of collagen results in the appearance of a series of characteristic absorptions which may be mis-assigned as DNA phosphate absorptions. The implications of the presence of collagen in tissues for the diagnosis of disease states by IR spectroscopic methods, with particular reference to cancer, is discussed.
本文展示了人类中枢神经系统组织和人类乳腺癌的红外光谱。根据组织的组成对光谱进行了讨论。结果表明，白质和灰质光谱之间的差异可以基于脂质含量的差异来解释。脑膜的脉络丛和蛛网膜绒毛的光谱显示出一系列在其他中枢神经系统组织中未观察到的吸收峰。根据样品的结缔组织含量对这些吸收峰进行了讨论。我们证明，胶原蛋白的存在会导致一系列特征吸收峰的出现，这些吸收峰可能会被误归为DNA磷酸盐吸收峰。本文还讨论了组织中胶原蛋白的存在对通过红外光谱法诊断疾病状态（特别是癌症）的影响。
