Anner B M, Imesch E, Moosmayer M
Laboratory of Experimental Cell Therapeutics, Geneva University Medical School, Switzerland.
Biochim Biophys Acta. 1995 Jan 25;1270(1):95-9. doi: 10.1016/0925-4439(94)00078-5.
Genetically hypertensive rats are excellent animal models for investigating putative Na+/K(+)-ATPase alterations associated with the disease. Highly purified Na+/K(+)-ATPase preparations from these animals have not yet been examined. Na+/K(+)-ATPases of two strains of spontaneously hypertensive rats, the Milan hypertensive strain (MHS) and the spontaneously hypertensive rat (SHR) were characterized in comparison with enzymes isolated from their matched normotensive controls; the sensitivity to Na ions as well as the shape and span of the inhibition curves for ouabain and mercury of the isolated Na+/K(+)-ATPases were compared. No functional changes between the purified 'normotensive' and 'hypertensive' Na+/K(+)-ATPases from brain and kidney were detected ruling out drastic structural alterations of the transport system in these two organs of diseased animals.
遗传性高血压大鼠是研究与该疾病相关的假定钠钾ATP酶改变的优秀动物模型。尚未对这些动物高度纯化的钠钾ATP酶制剂进行检测。对两种自发性高血压大鼠品系，即米兰高血压品系（MHS）和自发性高血压大鼠（SHR）的钠钾ATP酶进行了表征，并与从匹配的正常血压对照中分离的酶进行了比较；比较了分离的钠钾ATP酶对钠离子的敏感性以及哇巴因和汞抑制曲线的形状和跨度。未检测到来自脑和肾的纯化“正常血压”和“高血压”钠钾ATP酶之间的功能变化，排除了患病动物这两个器官中转运系统的剧烈结构改变。
