McDaniel M L, Roth C E, Fink C J, Lacy P E
Endocrinology. 1976 Aug;99(2):535-40. doi: 10.1210/endo-99-2-535.
The in vitro inhibition of insulin released by alloxan (20 mg/100 ml) in collagenase isolated rat islets is preferentially prevented by alpha D-glucose at a concentration of 1.0 mg/ml, while at a higher anomer concentration (1.5 mg/ml) both alpha and beta D-glucose provide equal protection. The ability of alpha D-glucose compared with beta D-glucose to stimulate insulin release, in vitro, showed a similar dose-related response, as observed in the alloxan protective studies. Although, both alpha and beta D-glucose compete with mutorated D-glucose for transport into islet cells, neither anomer produced a significantly different degree of inhibition in the transport process. The shared alpha stereospecificity for D-glucose in protection against alloxan and in stimulating insulin secretion in these in vitro studies, suggest a common site of interaction which may involve the beta-cell membrane.
在胶原酶分离的大鼠胰岛中，1.0毫克/毫升浓度的α-D-葡萄糖可优先阻止四氧嘧啶（20毫克/100毫升）对胰岛素释放的体外抑制作用，而在较高的异头物浓度（1.5毫克/毫升）下，α-D-葡萄糖和β-D-葡萄糖提供同等程度的保护。如在四氧嘧啶保护研究中所观察到的，与β-D-葡萄糖相比，α-D-葡萄糖在体外刺激胰岛素释放的能力呈现出类似的剂量相关反应。尽管α-D-葡萄糖和β-D-葡萄糖都与变旋D-葡萄糖竞争进入胰岛细胞的转运过程，但两种异头物在转运过程中产生的抑制程度均无显著差异。在这些体外研究中，D-葡萄糖在防止四氧嘧啶损伤和刺激胰岛素分泌方面具有共同的α立体特异性，这表明存在一个可能涉及β细胞膜的共同相互作用位点。
