Johnson M C, Goodman R B, Kajikawa O, Wong V A, Mongovin S M, Martin T R
Medical Research Service, Seattle Veterans Affairs Medical Center, WA 98108.
Gene. 1994 Dec 30;151(1-2):337-8. doi: 10.1016/0378-1119(94)90684-x.
We cloned two rabbit GRO homologue cDNAs from a lipopolysaccharide-stimulated rabbit alveolar macrophage (AM) cDNA library. One cDNA contains the complete coding sequence for a new mature GRO protein, RabGRO, which shares 68, 78 and 70% amino-acid identity with human GRO-alpha, -beta and -gamma, respectively. The other cDNA contains previously unreported sequence encoding a second GRO protein, rabbit permeability factor 2. The two Rab GRO proteins share 93% identity. Northern analysis shows that Rab AM GRO expression is rapidly induced by lipopolysaccharide. These findings suggest that GRO chemokines may be important in the pulmonary inflammation that occurs with septic lung injury.
我们从脂多糖刺激的兔肺泡巨噬细胞（AM）cDNA文库中克隆了两个兔GRO同源cDNA。一个cDNA包含一种新的成熟GRO蛋白RabGRO的完整编码序列，该蛋白与人类GRO-α、-β和-γ的氨基酸同一性分别为68%、78%和70%。另一个cDNA包含编码第二种GRO蛋白（兔通透性因子2）的先前未报道的序列。这两种Rab GRO蛋白的同一性为93%。Northern分析表明，脂多糖可快速诱导Rab AM GRO表达。这些发现提示，GRO趋化因子在脓毒症肺损伤所致肺部炎症中可能起重要作用。
