Huang S, Paulauskis J D, Kobzik L
Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115.
Biochem Biophys Res Commun. 1992 Apr 30;184(2):922-9. doi: 10.1016/0006-291x(92)90679-f.
We have isolated and sequenced overlapping cDNA clones for rat KC*. The 0.93 kb cDNA has a single open reading frame of 288 nucleotides, and substantial sequence identity with the platelet-factor 4 family members mouse KC, hamster gro, and human gro. Using cloned cDNA as a probe, expression of KC mRNA in lavaged rat alveolar macrophages (AMs) increased after lipopolysaccharide (LPS) treatment. We also studied expression in vitro by a rat fetal lung fibroblast cell line, RFL-6. Expression of KC mRNA in RFL-6 cells increased after treatment with interleukin 1 or with conditioned medium from rat AMs treated with LPS.
