Characterization of the genome region encoding structural proteins of Lactobacillus delbrueckii subsp. lactis bacteriophage LL-H.

Two regions from the genome of the virulent Lactobacillus delbrueckii subsp. lactic bacteriophage LL-H were sequenced (2330 and 12939 bp; 44% of the 34.6-kb genome). Together with the previously sequenced region containing the major capsid protein-encoding gene (2498 bp), the sequence had 21 open reading frames (ORFs) on the main coding strand. Only two putative ORFs were detected on the complementary strand. The ORFs covered 93.2% of the sequence. All but four of the ORFs were preceded by a ribosome-binding site. Only four longer non-coding stretches of sequences (175-278 nucleotides (nt) in size) were present. The longest of the non-coding regions contained an A + T-rich sequence that is surrounded by eight perfect copies of an 8-nt sequence that is present both as direct and inverted repeats. This region could represent the origin of replication. All the previously mapped structural protein-encoding genes of phage LL-H were included in the sequence. Genes were identified for the following five proteins: gp19 (encoded by gene g17), gp58 (g71), gp61 (g57), gp75 (g70) and gp89 (g88). N-terminal amino-acid sequencing was performed on gp19 and gp75, and it was found that the N-terminal Met had been post-translationally removed from both proteins.