Mikkonen M, Dupont L, Alatossava T, Ritzenthaler P
Department of Biology, University of Oulu, Finland.
Appl Environ Microbiol. 1996 May;62(5):1847-51. doi: 10.1128/aem.62.5.1847-1851.1996.
The phage attachment site, attP, and the integrase-encoding gene, int, are sufficient to promote site-specific integration of the temperate phage mv4 genome into the chromosome of the Lactobacillus delbrueckii host (L. Dupont, B. Boizet-Bonhoure, M. Coddeville, F. Auvray, and P. Ritzenthaler, J. Bacteriol. 177:586--595, 1995). The mv4 genome region containing these elements was compared at the nucleotide and amino acid levels with that of the closely related virulent phage LL-H. Complex DNA rearrangements were identified; a truncated integrase gene and two sites homologous to the mv4 attP site were detected in the genome of the virulent phage LL-H. These observations suggest that the two phages derive from a common temperate ancestor.
噬菌体附着位点attP和整合酶编码基因int足以促进温和噬菌体mv4基因组特定位点整合到德氏乳杆菌宿主（L. Dupont、B. Boizet-Bonhoure、M. Coddeville、F. Auvray和P. Ritzenthaler，《细菌学杂志》177:586 - 595，1995年）的染色体中。将含有这些元件的mv4基因组区域在核苷酸和氨基酸水平上与密切相关的烈性噬菌体LL-H的相应区域进行了比较。发现了复杂的DNA重排；在烈性噬菌体LL-H的基因组中检测到一个截短的整合酶基因和两个与mv4 attP位点同源的位点。这些观察结果表明这两种噬菌体源自一个共同的温和噬菌体祖先。
