Tao Q, Ho F C, Loke S L, Srivastava G
Department of Pathology, University of Hong Kong.
Int J Cancer. 1995 Jan 27;60(3):315-20. doi: 10.1002/ijc.2910600306.
Seven cases of nasal lymphoma were studied to identify the lineage of Epstein-Barr virus (EBV)+ cells using dual-labelling methods. Five cases were phenotypically and genotypically of natural killer cell (NK) type with germ-line configuration of T-cell receptor (TcR) beta-chain gene and immunoglobulin heavy-chain joining region (IgJH) gene, with one case each of T- and B-cell type showing rearranged TcR beta or IgJH and lambda-light chain genes respectively. EBV genome was clonal in all these cases except in the B-cell case where its clonality was undeterminable. Using in situ hybridization (ISH) for EBV-encoded small nuclear RNA 1 and 2 (EBER), signal was detected in 45% to 88% of nucleated cells in the tumours. Immunostaining for EBV latent membrane protein-I (LMP) also revealed numerous LMP+ cells in 3/5 NK-type cases and the T- and B-cell cases. Using ISH for EBER combined with immunostaining for CD markers and double immunohistochemistry for LMP and CD markers, the predominant lineage of the EBV+ cells was identified as: CD2+CD3-CD19-CD20- CD45R0 +/- CD56+CD68- in the NK-type cases, CD2+CD3 +/- CD19-CD20- CD45R0+CD56-CD68- in the T-cell case and CD20+CD45R0-CD68- in the B-cell case, in agreement with the genotype and phenotype of each tumour. These results show that, in EBV+ nasal lymphomas of NK, T- or B-cell lineage, EBV was consistently associated with the tumour-cell population and support the view that EBV serves a promoting role in the pathogenesis of different types of EBV+ nasal lymphoma.
研究了7例鼻淋巴瘤，采用双标记法鉴定爱泼斯坦-巴尔病毒（EBV）阳性细胞的谱系。5例在表型和基因型上为自然杀伤细胞（NK）型，T细胞受体（TcR）β链基因和免疫球蛋白重链连接区（IgJH）基因为种系构型，T细胞型和B细胞型各1例，分别显示TcRβ或IgJH及λ轻链基因重排。除B细胞型病例中EBV克隆性无法确定外，所有这些病例中EBV基因组均为克隆性。使用原位杂交（ISH）检测EBV编码的小核RNA 1和2（EBER），在肿瘤中有核细胞的45%至88%中检测到信号。对EBV潜伏膜蛋白-I（LMP）进行免疫染色，在3/5的NK型病例以及T细胞型和B细胞型病例中也发现了大量LMP阳性细胞。使用针对EBER的ISH结合CD标志物免疫染色以及针对LMP和CD标志物的双重免疫组化，EBV阳性细胞的主要谱系被确定为：NK型病例中为CD2 + CD3 - CD19 - CD20 - CD45R0 +/- CD56 + CD68 -，T细胞型病例中为CD2 + CD3 +/- CD19 - CD20 - CD45R0 + CD56 - CD68 -，B细胞型病例中为CD20 + CD45R0 - CD68 -，与每个肿瘤的基因型和表型一致。这些结果表明，在NK、T或B细胞谱系的EBV阳性鼻淋巴瘤中，EBV始终与肿瘤细胞群体相关，并支持EBV在不同类型的EBV阳性鼻淋巴瘤发病机制中起促进作用的观点。
