Elenitoba-Johnson K S, Zarate-Osorno A, Meneses A, Krenacs L, Kingma D W, Raffeld M, Jaffe E S
Section of Hematopathology, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Mod Pathol. 1998 Aug;11(8):754-61.
Nasal T-lymphocyte/natural killer cell lymphomas (nT/NKLs) are a distinct group of neoplasms highly associated with Epstein-Barr virus (EBV), with a high prevalence in Asia but rare in Western countries. Recent studies indicate that these neoplasms are of cytotoxic T- or NK-cell derivation. Previous studies identifying a characteristic 30-base pair deletion within the 3' end of latent membrane protein-1 (del-LMP-1) in other EBV-associated lymphomas suggested a pathogenetic role for del-LMP-1 in those neoplasms. We examined 23 cases of nT/NKL from Mexico for expression of the cytolytic granular proteins TIA-1 and perforin (PRF), and for the presence of EBV by in situ hybridization (ISH). Polymerase chain reaction was performed to identify the EBV (EBNA-2) strain type and the status of the LMP-1 gene (del-LMP-1). Controls consisted of 11 sinonasal B-cell lymphomas (nBLs) and 30 reactive tonsils (RTs) from healthy Mexican individuals. The nT/NKLs expressed TIA-1 in 21 (91%) of 23 cases and PRF in 15 (65%) of 23 cases. In contrast, all of the nBLs were negative for TIA-1 and PRF. Twenty-two (96%) of 23 nT/NKLs were positive for EBV by ISH. In contrast, only 2 (18%) of 11 nBLs were positive for EBV by ISH. EBV strain Type A was identified in 21 (91%) of 23 cases, whereas strain Type B was present in 2 (9%) of the 23 nT/NKLs. A similar percentage (80%) of Type A was noted in 12 of the 15 RTs. del-LMP-1 was detected in 6 (26%) of 23 nT/NKLs, comprising 4 cases of Type A and 2 of Type B. del-LMP-1 was detected in 9 (45%) of 20 RTs. Our results indicated that TIA-1 and PRF were sensitive markers of nT/NKL. The presence of del-LMP-1 in comparable frequencies in the RTs and nT/NKLs suggested to us that this genotype was common in the Mexican population and argued against a definite pathogenetic role for del-LMP-1 in nT/NKL.
鼻T淋巴细胞/自然杀伤细胞淋巴瘤（nT/NKL）是一组与爱泼斯坦-巴尔病毒（EBV）高度相关的独特肿瘤，在亚洲发病率高，而在西方国家罕见。最近的研究表明，这些肿瘤起源于细胞毒性T细胞或NK细胞。先前的研究在其他EBV相关淋巴瘤的潜伏膜蛋白-1（del-LMP-1）3'端发现了一个特征性的30碱基对缺失，提示del-LMP-1在这些肿瘤中具有致病作用。我们检测了来自墨西哥的23例nT/NKL病例，以检测其溶细胞颗粒蛋白TIA-1和穿孔素（PRF）的表达，并通过原位杂交（ISH）检测EBV的存在情况。采用聚合酶链反应来鉴定EBV（EBNA-2）毒株类型和LMP-1基因（del-LMP-1）的状态。对照组包括11例鼻窦B细胞淋巴瘤（nBL）和30例来自健康墨西哥人的反应性扁桃体（RT）。23例nT/NKL中有21例（91%）表达TIA-1，23例中有15例（65%）表达PRF。相比之下，所有nBL的TIA-1和PRF均为阴性。23例nT/NKL中有22例（96%）ISH检测EBV呈阳性。相比之下，11例nBL中只有2例（18%）ISH检测EBV呈阳性。23例中有21例（91%）鉴定为EBV A型毒株，23例nT/NKL中有2例（9%）为B型毒株。15例RT中有12例（80%）为A型。23例nT/NKL中有6例（26%）检测到del-LMP-1，其中4例为A型，2例为B型。20例RT中有9例（45%）检测到del-LMP-1。我们的结果表明，TIA-1和PRF是nT/NKL的敏感标志物。RT和nT/NKL中del-LMP-1出现频率相当，这表明该基因型在墨西哥人群中很常见，也反驳了del-LMP-1在nT/NKL中具有明确致病作用的观点。
