Schaefer D, Jürgensmeier J M, Bauer G
Department of Virology, University of Freiburg, Germany.
Int J Cancer. 1995 Feb 8;60(4):520-6. doi: 10.1002/ijc.2910600416.
We have recently shown that TGF-beta-treated normal fibroblasts are able to induce apoptosis of transformed fibroblasts, leading to their elimination. Here we describe a test system that allows the quantitative analysis of the elimination of G418-resistant transformed cells by TGF-beta-treated normal cells. This assay system was used to screen for substances that interfere with the elimination of transformed cells. Catechol and hydroquinone, but not resorcinol, were found to represent potent antagonists of TGF-beta-induced elimination of transformed cells by normal cells. Protection of transformed cells from negative effects derived from their cellular environment defines a hitherto unrecognized crucial mechanism for the survival of transformed cells. The protective effect of catechol as seen in this experimental system may act in concert with its co-carcinogenic and promoting activities during carcinogenesis.
我们最近发现，经转化生长因子-β（TGF-β）处理的正常成纤维细胞能够诱导转化成纤维细胞凋亡，从而使其被清除。在此，我们描述了一种测试系统，该系统可对经TGF-β处理的正常细胞清除G418抗性转化细胞的过程进行定量分析。此检测系统用于筛选干扰转化细胞清除的物质。结果发现，儿茶酚和对苯二酚（而非间苯二酚）是TGF-β诱导正常细胞清除转化细胞的有效拮抗剂。保护转化细胞免受其细胞环境产生的负面影响，定义了一种迄今未被认识到的转化细胞存活关键机制。在该实验系统中观察到的儿茶酚的保护作用，可能在致癌过程中与其促癌和促进活性协同发挥作用。
