Hube B, Monod M, Schofield D A, Brown A J, Gow N A
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, UK.
Mol Microbiol. 1994 Oct;14(1):87-99. doi: 10.1111/j.1365-2958.1994.tb01269.x.
The opportunistic fungal pathogen Candida albicans produces secretory aspartyl proteinases, which are believed to be virulence factors in infection. We have studied the in vitro expression of seven known members of the SAP gene family in a range of strains and serotypes by Northern analysis. SAP1 and SAP3 were regulated during phenotypic switching between the white and opaque forms of the organism. The SAP2 mRNA, which was the dominant transcript in the yeast form, was found to be autoinduced by peptide products of Sap2 activity and to be repressed by amino acids. The expression of the closely related SAP4-SAP6 genes was observed only at neutral pH during serum-induced yeast to hyphal transition. No SAP7 mRNA was detected under any of the conditions or in any of the strains tested. Our data suggest that the various members of the SAP gene family may have distinct roles in the colonization and invasion of the host.
机会性真菌病原体白色念珠菌可产生分泌性天冬氨酸蛋白酶，这些蛋白酶被认为是感染中的毒力因子。我们通过Northern分析研究了一系列菌株和血清型中SAP基因家族七个已知成员的体外表达情况。在该生物体白色和不透明形态之间的表型转换过程中，SAP1和SAP3受到调控。SAP2 mRNA是酵母形态中的主要转录本，发现其受Sap2活性的肽产物自动诱导，并被氨基酸抑制。仅在血清诱导酵母向菌丝转变过程中的中性pH条件下观察到密切相关的SAP4 - SAP6基因的表达。在任何测试条件下或任何测试菌株中均未检测到SAP7 mRNA。我们的数据表明，SAP基因家族的各个成员在宿主的定殖和侵袭中可能具有不同的作用。
