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促胰液素在胰腺单层培养物中的免疫荧光定位。

Immunofluorescent localization of secretin in pancreatic monolayer culture.

作者信息

Rufener C, Amherdt M, Baetens D, Yanaihara N, Orci L

出版信息

Histochemistry. 1976 May 28;47(2):171-3. doi: 10.1007/BF00492564.

DOI:10.1007/BF00492564
PMID:783098
Abstract

Immunofluorescent cells to synthetic secretin were identified in monolayer culture of neonatal rat pancreas. No cross reaction of anti-secretin was observed with either glucagon, somatostatin or gastrin. The presence of cells containing secretin or a secretin-like peptide adds a new cell type to the three already characterized (insulin, glucagon and somatostatin containing cells) in monolayer culture.

摘要

在新生大鼠胰腺单层培养物中鉴定出对合成促胰液素呈免疫荧光反应的细胞。未观察到抗促胰液素与胰高血糖素、生长抑素或胃泌素发生交叉反应。含有促胰液素或促胰液素样肽的细胞的存在,为单层培养中已鉴定的三种细胞类型(含胰岛素、胰高血糖素和生长抑素的细胞)增添了一种新的细胞类型。

相似文献

1
Immunofluorescent localization of secretin in pancreatic monolayer culture.促胰液素在胰腺单层培养物中的免疫荧光定位。
Histochemistry. 1976 May 28;47(2):171-3. doi: 10.1007/BF00492564.
2
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Aust J Biol Sci. 1985;38(1):59-66.

引用本文的文献

1
Identification of a transcriptional enhancer important for enteroendocrine and pancreatic islet cell-specific expression of the secretin gene.鉴定一种对促胰液素基因的肠内分泌和胰岛细胞特异性表达至关重要的转录增强子。
Mol Cell Biol. 1992 Aug;12(8):3531-9. doi: 10.1128/mcb.12.8.3531-3539.1992.
2
Immunoreactivity of the endocrine pancreas. Evidence for the presence of cholecystokinin- pancreozymin within the A-cell.内分泌胰腺的免疫反应性。A细胞内存在胆囊收缩素-促胰酶素的证据。
Histochemistry. 1978 Jun 2;56(1):13-35. doi: 10.1007/BF00492250.

本文引用的文献

1
Immunofluorescent localization of secretin in the canine duodenum.免疫荧光定位法在犬十二指肠分泌素的研究。
Gut. 1971 Aug;12(8):605-10. doi: 10.1136/gut.12.8.605.
2
Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.抗体产生的研究。I. 一种特异性抗体组织化学显示方法及其在超免疫兔研究中的应用。
J Exp Med. 1955 Jul 1;102(1):49-60. doi: 10.1084/jem.102.1.49.
3
Synthesis of a heptacosapeptide amide with the hormonal activity of sccretin.
具有促胰液素激素活性的二十七肽酰胺的合成。
Chem Ind. 1966 Oct 15;42:1757-8.
4
Monolayer cell culture of neonatal rat pancreas: light microscopy and evidence for immunoreactive insulin synthesis and release.新生大鼠胰腺的单层细胞培养:光学显微镜检查及免疫反应性胰岛素合成与释放的证据。
Endocrinology. 1972 Jan;90(1):239-48. doi: 10.1210/endo-90-1-239.
5
Enzymic iodination of the histidyl residue of secretin: a radioimmunoassay of the hormone.促胰液素组氨酸残基的酶促碘化作用:该激素的放射免疫测定法
Biochim Biophys Acta. 1973 Sep 21;322(1):178-80. doi: 10.1016/0005-2795(73)90190-6.
6
Immunohistochemical and ultrastructural detection of the secretin cell in the pig intestinal mucosa.猪肠黏膜中促胰液素细胞的免疫组织化学和超微结构检测
Experientia. 1972 May 15;28(5):549-50. doi: 10.1007/BF01931870.
7
Identification of the diarrheogenic hormone associated with non-beta islet cell tumors of the pancreas.与胰腺非β胰岛细胞瘤相关的致腹泻激素的鉴定。
Ann Surg. 1968 Sep;168(3):502-21. doi: 10.1097/00000658-196809000-00016.
8
Islet cell tumor of the pancreas with reversible watery diarrhea and achylorhydraia.胰腺胰岛细胞瘤伴可逆性水样腹泻和无胃酸症。
Gastroenterology. 1967 Apr;52(4):695-708.
9
Pancreatic endocrine tumors--the riddle or their origin and hormone secretion.胰腺内分泌肿瘤——其起源与激素分泌之谜
Isr J Med Sci. 1975 Jul;11(7):762-76.