Cox M E, Mangels J I
J Clin Microbiol. 1976 Jul;4(1):40-5. doi: 10.1128/jcm.4.1.40-45.1976.
A small portable chamber for the recovery of anaerobic bacteria is described. This rigid chamber is constructed of clear acrylic with dimensions of 30 inches (ca. 76.2 cm) wide, 18 inches (ca. 44.7 cm) deep, and 18 inches (ca. 44.7 cm) high. Conventional bacteriological techniques can be used inside the chamber to efficiently isolate strict anaerobic organisms. An adapter allows the attachment of a standard anaerobic jar to the outside of the chamber. The jar can be used to store reduced media. Once the jar is attached to the chamber and the media is removed to the interior of the chamber, the jar is available to receive inoculated media. The anaerobic jar can then be removed from the chamber, without contaminating the jar or chamber with oxygen, and be placed in a conventional 37degreesC incubator. This chamber also allows the microbiologist to process cultures without wearing gloves as was necessary with previous anaerobic chambers. Air-tight latex rubber sleeves seal around the microbiologists arms and to the armport flange of the chamber to prevent the introduction of oxygen into the chamber. Anaerobic conditions are maintained by circulating a 80% N2, 10% H2, 10% CO2 gas mixture through alumina pellets coated with palladium. This study indicates that anaerobic conditions obtained in this chamber are sufficient for recovery of obligate anaerobes.
描述了一种用于厌氧细菌复苏的小型便携式培养箱。这个刚性培养箱由透明丙烯酸制成，宽30英寸（约76.2厘米），深18英寸（约44.7厘米），高18英寸（约44.7厘米）。可在培养箱内使用常规细菌学技术有效分离严格厌氧生物。一个适配器可将标准厌氧罐连接到培养箱外部。该罐可用于储存还原培养基。一旦将罐连接到培养箱并将培养基转移到培养箱内部，该罐就可用于接收接种后的培养基。然后可将厌氧罐从培养箱中取出，而不会使罐或培养箱被氧气污染，并可放置在传统的37℃培养箱中。该培养箱还使微生物学家在处理培养物时无需像以前的厌氧培养箱那样戴手套。气密乳胶橡胶套筒围绕微生物学家的手臂密封并连接到培养箱的手臂端口法兰，以防止氧气进入培养箱。通过使80%N2、10%H2、10%CO2的气体混合物通过涂有钯的氧化铝颗粒循环来维持厌氧条件。本研究表明，在该培养箱中获得的厌氧条件足以复苏专性厌氧菌。
