Avellaneda G E, Villegas P, Jackwood M W, King D J
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-4875.
Avian Dis. 1994 Jul-Sep;38(3):589-97.
The pathogenicity of 13 field isolates of infectious bronchitis virus (IBV) isolated from Georgia broiler farms from 1989 to 1992 was evaluated using the IBV and Escherichia coli mixed-infection model. Based on the clinical signs, mortality, and lesions, the isolates were classified as high, intermediate, and low in pathogenicity. The in vivo classification was compared with the serotype classification results obtained by reverse transcriptase-polymerase chain reaction-restriction fragment length polymorphism analysis. The high-pathogenicity group was composed of five isolates representing three serotypes: Arkansas, Georgia variant (GAV), and Massachusetts. Isolates in the intermediate- and low-pathogenicity groups were all representatives of the Connecticut serotype, except for one isolate, which belonged to the Massachusetts serotype.
利用传染性支气管炎病毒（IBV）与大肠杆菌混合感染模型，对1989年至1992年从格鲁吉亚肉鸡场分离出的13株IBV野毒株的致病性进行了评估。根据临床症状、死亡率和病变情况，将这些分离株分为高致病性、中等致病性和低致病性。将体内分类结果与通过逆转录-聚合酶链反应-限制性片段长度多态性分析获得的血清型分类结果进行了比较。高致病性组由代表三种血清型的五株分离株组成：阿肯色型、格鲁吉亚变异株（GAV）和马萨诸塞型。中等致病性和低致病性组的分离株均为康涅狄格血清型的代表，只有一株属于马萨诸塞血清型。
