部分肝切除大鼠的胆汁分泌与肝再生

Bile secretion and liver regeneration in partially hepatectomized rats.

作者信息

Xu H S, Rosenlof L K, Jones R S

机构信息

Department of Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.

出版信息

Ann Surg. 1993 Aug;218(2):176-82. doi: 10.1097/00000658-199308000-00009.

DOI:10.1097/00000658-199308000-00009

PMID:8342997

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1242927/

Abstract

OBJECTIVE

The purpose of this research was to study the correlation between bile secretion and the liver regeneration in the partially hepatectomized rat.

SUMMARY BACKGROUND DATA

Significant alteration in bile formation and secretion is expected in the liver after hepatectomy. There is scant literature, however, about the effects of liver regeneration in bile secretion.

METHODS

The work was done in rats with 50% hepatectomy, 75% hepatectomy, and sham operation as the control. A chronic common bile duct fistula and a duodenal cannula were established for bile collection and the sample analysis on days 1, 3, 5, 7, and 9.

RESULTS

With size reduced in the liver after 50% and 75% hepatectomy, the total bile volume decreased 45.9% and 51.5%, bile salt independent flow decreased 59.3% and 64.9%, bile salt secretion rate decreased 36.1% and 43.4%, bile salt basal synthesis rate decreased 52.3% and 56.4%, phospholipid secretion rate decreased 52.6% and 68.0%, and cholesterol secretion rate decreased 54.3% and 72.4% from control on day 1, respectively. All changes returned to the control level in 3 to 9 days with accompanying increasing liver size during regeneration.

CONCLUSION

Alterations of total bile flow, bile salt independent flow, bile salt secretion rate, bile salt basal synthesis rate, and biliary lipid secretion after partial hepatectomy correlate with the liver regeneration rate in rats. Partial hepatectomy reduces the bile salt independent fraction calculated as per 100 g body weight rather than the dependent fraction. The study of bile salt and biliary lipid secretion is a useful method for monitoring synthetic function in liver regeneration in vivo.

摘要

目的

本研究旨在探讨部分肝切除大鼠胆汁分泌与肝再生之间的相关性。

总结背景资料

肝切除术后肝脏胆汁生成和分泌预计会发生显著改变。然而，关于肝再生对胆汁分泌影响的文献却很少。

方法

本研究以50%肝切除、75%肝切除的大鼠及假手术组大鼠作为对照。通过建立慢性胆总管瘘和十二指肠插管，于术后第1、3、5、7和9天收集胆汁并进行样本分析。

结果

50%和75%肝切除术后肝脏体积减小，术后第1天，总胆汁量分别较对照组减少45.9%和51.5%，胆盐非依赖性胆汁流分别减少59.3%和64.9%，胆盐分泌率分别减少36.1%和43.4%，胆盐基础合成率分别减少52.3%和56.4%，磷脂分泌率分别减少52.6%和68.0%，胆固醇分泌率分别减少54.3%和72.4%。随着肝脏在再生过程中体积增大，所有变化在3至9天内恢复至对照水平。