Sancho P, Mendieta J, García-Pérez A I, Luque J
Departamento de Bioquímica y Biología Molecular Universidad de Alcalá, Campus Universitario, Madrid, Spain.
Biosci Rep. 1994 Jun;14(3):119-30. doi: 10.1007/BF01240244.
Rat bone marrow cell populations, containing different proportions of erythroid cells, have been fractionated by counter-current distribution in the non-charge-sensitive dextran/polyethyleneglycol two-phase systems on the basis of hydrophobic cell surface properties. Cell fractions with a low distribution coefficient, which contain non-erythroid cells and early erythoblasts, showed a low transferrin binding capacity and a low haemoglobin/cell ratio whereas cell fractions with a high distribution coefficient, which contain intermediate-late erythroblasts and mature red cells, showed an elevated transferrin binding capacity and the highest haemoglobin/cell ratio. These results support transferrin binding capacity as a good marker parameter for the erythroid bone marrow cell differentiation and maturation processes.
含有不同比例红细胞的大鼠骨髓细胞群体，已根据细胞表面疏水特性，在对电荷不敏感的葡聚糖/聚乙二醇双相系统中通过逆流分配进行了分级分离。分配系数低的细胞级分含有非红细胞和早期成红细胞，其转铁蛋白结合能力低，血红蛋白/细胞比率也低；而分配系数高的细胞级分含有中晚期成红细胞和成熟红细胞，其转铁蛋白结合能力升高，血红蛋白/细胞比率最高。这些结果支持转铁蛋白结合能力作为红细胞系骨髓细胞分化和成熟过程的良好标记参数。
