Zhao Baobing, Mei Yang, Yang Jing, Ji Peng
Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Exp Hematol. 2016 Oct;44(10):975-81. doi: 10.1016/j.exphem.2016.06.249. Epub 2016 Jun 27.
Differentiating erythroblasts are exposed to an oxidative environment. The dynamics of oxidative status during terminal erythropoiesis and how they affect cell differentiation in response to erythropoietin (Epo) are unclear. Here, we show that Epo induces reactive oxygen species (ROS) production in the early stages of terminal erythropoiesis. The levels of ROS correlate with CD71 surface expression and the uptake of iron and transferrin. ROS decreases in the late stages of terminal erythropoiesis, when the cells are preparing for enucleation. Consistently, treatment of erythroblasts with a low dose (5 mM) of N-acetyl-cysteine (NAC), a ROS scavenger, promotes enucleation. However, a high dose (20 mM) of NAC leads to significant cell death. Our study reveals an important function of Epo in regulating the dynamics of oxidative status and enucleation.
正在分化的成红细胞处于氧化环境中。终末红细胞生成过程中氧化状态的动态变化以及它们如何响应促红细胞生成素（Epo）影响细胞分化尚不清楚。在此，我们表明Epo在终末红细胞生成的早期阶段诱导活性氧（ROS）的产生。ROS水平与CD71表面表达以及铁和转铁蛋白的摄取相关。当细胞准备去核时，ROS在终末红细胞生成的后期阶段减少。一致地，用低剂量（5 mM）的ROS清除剂N-乙酰半胱氨酸（NAC）处理成红细胞可促进去核。然而，高剂量（20 mM）的NAC会导致显著的细胞死亡。我们的研究揭示了Epo在调节氧化状态和去核动态方面的重要功能。
