一种用于定量生物样品中人类酰基辅酶A：甘氨酸N-酰基转移酶的免疫检测方法。

An immunodetection method for the quantitation of human acyl CoA:glycine N-acyltransferase in biological samples.

作者信息

Mawal Y R, Qureshi I A

机构信息

Centre de recherche, Hôpital Sainte-Justine, Montréal, Québec, Canada.

出版信息

Biochem Mol Biol Int. 1994 Oct;34(3):595-601.

PMID:7833837

Abstract

An immunochemical method for quantitation of human acyl CoA:glycine N-acyltransferase (ACGNAT) has been developed. A 33 kD ovine ACGNAT was purified to homogeneity and was used to raise polyclonal antibodies in rabbits. These antibodies were then employed for developing a sensitive immunodiagnostic method as they cross-reacted with human ACGNAT. Using this methodology, a linear correlation was seen between specific activity and ACGNAT concentration in an ammonium sulphate precipitation fraction (40-60%) of hepatic tissues of children and adults of 4 h age to 11 year old (r = 0.99, n = 6).

摘要

已开发出一种用于定量人酰基辅酶A：甘氨酸N-酰基转移酶（ACGNAT）的免疫化学方法。将一种33kD的绵羊ACGNAT纯化至同质，并用于在兔中产生多克隆抗体。这些抗体随后被用于开发一种灵敏的免疫诊断方法，因为它们与人类ACGNAT发生交叉反应。使用该方法，在4小时至11岁儿童和成人肝脏组织的硫酸铵沉淀级分（40-60%）中，比活性与ACGNAT浓度之间呈线性相关（r = 0.99，n = 6）。