胆碱能刺激期间冷却对兔耳动脉和股动脉一氧化氮生成的影响。

Cooling effects on nitric oxide production by rabbit ear and femoral arteries during cholinergic stimulation.

作者信息

Fernández N, Monge L, García-Villalón A L, García J L, Gómez B, Diéguez G

机构信息

Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.

出版信息

Br J Pharmacol. 1994 Oct;113(2):550-4. doi: 10.1111/j.1476-5381.1994.tb17024.x.

DOI:10.1111/j.1476-5381.1994.tb17024.x

PMID:7834207

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1510108/

Abstract

Ear (cutaneous) and femoral (deep) arteries from rabbit were perfused at 37 degrees C and 24 degrees C (cooling) and the production of nitrite, as an index of nitric oxide production, was measured under basal conditions and cholinergic stimulation. 2. In both types of arteries under control conditions, the basal production of nitrite was similar at 24 degrees C and 37 degrees C. Compared with the control conditions, the basal production of nitrite was significantly lower in ear and femoral arteries without endothelium or treated with NG-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) but it was similar in those treated with atropine (10(-6) M). 3. At 37 degrees C, methacholine (10(-7)-10(-5) M) increased the production of nitrite in ear and femoral arteries; this increase persisted during 30-60 min and was practically abolished by L-NAME (10(-4) M), atropine (10(-6) M), or removal of the endothelium. In ear arteries the total nitrite production to activation with methacholine was higher at 24 degrees C than at 37 degrees C due to this production persisted increased for a longer period (> 150 min), whereas in femoral arteries it was lower at 24 degrees C than at 37 degrees C. 4. It is suggested that: (a) the endothelium of rabbit ear and femoral arteries produce nitric oxide under basal conditions, which is increased by cholinergic stimulation, and (b) cooling potentiates endothelial nitric oxide production to cholinergic stimulation in cutaneous arteries, whereas it inhibits this production in deep arteries.

摘要

将兔的耳（皮肤）动脉和股（深部）动脉分别在37℃和24℃（冷却）下进行灌注，并在基础条件和胆碱能刺激下测量亚硝酸盐的生成量，以此作为一氧化氮生成的指标。2. 在对照条件下，两种类型的动脉在24℃和37℃时亚硝酸盐的基础生成量相似。与对照条件相比，无内皮或用NG-硝基-L-精氨酸甲酯（L-NAME，10⁻⁴ M）处理的耳动脉和股动脉中，亚硝酸盐的基础生成量显著降低，但用阿托品（10⁻⁶ M）处理的动脉中则相似。3. 在37℃时，乙酰甲胆碱（10⁻⁷ - 10⁻⁵ M）可增加耳动脉和股动脉中亚硝酸盐的生成；这种增加在30 - 60分钟内持续存在，并且几乎被L-NAME（10⁻⁴ M）、阿托品（10⁻⁶ M）或去除内皮所消除。在耳动脉中，由于乙酰甲胆碱激活后亚硝酸盐的总生成量在24℃时比在37℃时更高，因为这种生成量持续增加的时间更长（> 150分钟），而在股动脉中，24℃时则比37℃时更低。4. 由此表明：（a）兔耳动脉和股动脉的内皮在基础条件下产生一氧化氮，胆碱能刺激可使其增加；（b）冷却增强了皮肤动脉中内皮对胆碱能刺激产生一氧化氮的能力，而在深部动脉中则抑制这种生成。