冷却过程中内皮在兔耳动脉和股动脉对胆碱能受体刺激反应中的作用

Role of the endothelium in the response to cholinoceptor stimulation of rabbit ear and femoral arteries during cooling.

作者信息

Monge L, García-Villalón A L, Montoya J J, García J L, Fernández N, Gómez B, Diéguez G

机构信息

Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.

出版信息

Br J Pharmacol. 1993 May;109(1):61-7. doi: 10.1111/j.1476-5381.1993.tb13531.x.

DOI:10.1111/j.1476-5381.1993.tb13531.x

PMID:8495247

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2175572/

Abstract

The role of the endothelium in the effects of cooling on the response to cholinoceptor stimulation of the rabbit central ear (cutaneous) and femoral (non-cutaneous) arteries was studied using 2 mm long cylindrical segments. 2. Concentration-response curves for acetylcholine (10(-9)-10(-5) M), methacholine (10(-9)-10(-5) M) and sodium nitroprusside (10(-9)-10(-4) M) were isometrically recorded in arteries under conditions, with and without endothelium or following pretreatment with the nitric oxide-synthesis inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 10(-6)-3 x 10(-4) M) at 37 degrees C and at 24 degrees C (cooling). 3. Ear and femoral arteries showed endothelium-dependent relaxation to acetylcholine and methacholine at 37 degrees C and 24 degrees C. The extent of relaxation of the control ear arteries, but not of the control femoral arteries, to acetylcholine and methacholine increased during cooling. 4. L-NAME (10(-6)-3 x 10(-4) M) reduced in a concentration-dependent way the response of ear arteries to acetylcholine at both 37 degrees C and 24 degrees C, this reduction being more potent at 37 degrees C. L-Arginine (10(-5)-10(-3) M) reversed in a concentration-dependent manner the inhibitor effects of 10(-5) M L-NAME at both temperatures. 5. Sodium nitroprusside caused a concentration-dependent relaxation in both arteries that was endothelium-independent. However, the extent of relaxation to this nitrovasodilator in ear and femoral arteries was lower at 24 degrees C. 6. These results suggest that cooling augments the reactivity of cutaneous (ear) arteries, but not that of non-cutaneous (femoral) arteries to cholinoceptor stimulation by endothelium-mediated mechanisms.Cooling could therefore facilitate the stimulated release of endothelial nitric oxide in cutaneous vessels.

摘要

使用2毫米长的圆柱形节段，研究了内皮在冷却对兔中耳（皮肤）和股动脉（非皮肤）对胆碱能受体刺激反应的影响中的作用。2. 在有或无内皮的条件下，或在37℃和24℃（冷却）下用一氧化氮合成抑制剂NG-硝基-L-精氨酸甲酯（L-NAME，10(-6)-3×10(-4)M）预处理后，等长记录乙酰胆碱（10(-9)-10(-5)M）、醋甲胆碱（10(-9)-10(-5)M）和硝普钠（10(-9)-10(-4)M）的浓度-反应曲线。3. 耳动脉和股动脉在37℃和24℃时对乙酰胆碱和醋甲胆碱表现出内皮依赖性舒张。冷却期间，对照耳动脉对乙酰胆碱和醋甲胆碱的舒张程度增加，而对照股动脉则未增加。4. L-NAME（10(-6)-3×10(-4)M）在37℃和24℃时均以浓度依赖性方式降低耳动脉对乙酰胆碱的反应，这种降低在37℃时更显著。L-精氨酸（10(-5)-10(-3)M）在两个温度下均以浓度依赖性方式逆转10(-5)M L-NAME的抑制作用。5. 硝普钠在两条动脉中均引起浓度依赖性舒张，且不依赖于内皮。然而，在24℃时，耳动脉和股动脉对这种硝基血管扩张剂的舒张程度较低。6. 这些结果表明，冷却增强了皮肤（耳）动脉对胆碱能受体刺激的反应性，但未增强非皮肤（股）动脉的反应性，其机制是内皮介导的。因此，冷却可能促进皮肤血管中内皮一氧化氮的刺激释放。