Rowse G J, Weinberg J, Emerman J T
Department of Anatomy, University of British Columbia, Vancouver, Canada.
Cancer Res. 1995 Feb 1;55(3):617-22.
We have shown that social housing conditions affect the growth rate of the androgen-responsive Shionogi mouse mammary tumor (SC115) and differentially stimulate splenic natural killer (NK) cell activity. Mice reared in a social group and then singly housed (GI) following tumor cell injection have increased tumor growth rates and increased NK cell activity, whereas mice reared individually and then group housed following tumor cell injection have decreased tumor growth rates and decreased NK cell activity compared to that in mice remaining in their rearing group. The present study was undertaken to determine whether NK cells are involved in mediating the effects of social housing conditions on SC115 tumor growth rates. We demonstrate that the presence of the SC115 tumor significantly stimulates the activity of NK cells at the tumor site in the first 7 days after tumor cell injection, and that, consistent with the data on splenic NK cells, mice of the GI group (largest tumors) have significantly greater levels of NK cell activity than mice reared individually and then group housed (smallest tumors). We further demonstrate that in mice of the GI group, in vivo stimulation of NK cell activity by polyinosinic:polyCMP correlates with a corresponding increase in tumor growth rate. These findings suggest that NK cell activity may play a role in mediating the increased tumor growth rate observed in mice of the GI housing condition.
我们已经表明，社会住房条件会影响雄激素反应性的狮王小鼠乳腺肿瘤（SC115）的生长速度，并差异性地刺激脾脏自然杀伤（NK）细胞的活性。在肿瘤细胞注射后，先在群居环境中饲养然后单独饲养（GI）的小鼠，肿瘤生长速度加快，NK细胞活性增强；而先单独饲养然后在肿瘤细胞注射后群居的小鼠，与仍留在饲养群体中的小鼠相比，肿瘤生长速度减慢，NK细胞活性降低。本研究旨在确定NK细胞是否参与介导社会住房条件对SC115肿瘤生长速度的影响。我们证明，SC115肿瘤的存在在肿瘤细胞注射后的前7天显著刺激了肿瘤部位NK细胞的活性，并且与脾脏NK细胞的数据一致，GI组（肿瘤最大）的小鼠NK细胞活性水平显著高于先单独饲养然后群居（肿瘤最小）的小鼠。我们进一步证明，在GI组小鼠中，聚肌苷酸:聚胞苷酸对NK细胞活性的体内刺激与肿瘤生长速度的相应增加相关。这些发现表明，NK细胞活性可能在介导GI住房条件下小鼠观察到的肿瘤生长速度增加中发挥作用。
