Sellar G C, Oghene K, Boyle S, Bickmore W A, Whitehead A S
Department of Genetics, Trinity College, University of Dublin, Ireland.
Genomics. 1994 Sep 15;23(2):492-5. doi: 10.1006/geno.1994.1530.
The four members of the human serum amyloid A protein (SAA) gene family are clustered on human chromosome 11p15.1. Three genes are differentially expressed and encode small apolipoproteins of M(r) 12-19 kDa: SAA1 and SAA2 encode the acute phase SAAs (A-SAAs), and SAA4 encodes the constitutively expressed SAA (C-SAA). A fourth locus, SAA3, is a pseudogene. The human SAA gene family encompasses approximately 150 kb contained on a 900-kb yeast artificial chromosome contig. SAA1 and SAA2 are 15-20 kb apart and are arranged in divergent transcriptional orientations. SAA4 is 9 kb downstream of SAA2 and in the same orientation. SAA3 is 110 kb downstream of SAA4, and its relative orientation could not be determined. All genes known to be in the same human and mouse syntenic linkage group as SAA were mapped within the contig. Interphase FISH was used to orientate the region relative to the centromere: cen-LDHC-LDHA-SAA1-SAA2-SAA4-SAA3-TP H-D11S18- KCNC1-MYOD1-pter.
人血清淀粉样蛋白A蛋白（SAA）基因家族的四个成员聚集在人类11号染色体的11p15.1区域。其中三个基因呈差异表达，编码分子量为12 - 19 kDa的小载脂蛋白：SAA1和SAA2编码急性期SAA（A - SAA），SAA4编码组成型表达的SAA（C - SAA）。第四个基因座SAA3是一个假基因。人类SAA基因家族包含在一个900 kb的酵母人工染色体重叠群上的约150 kb片段。SAA1和SAA2相距15 - 20 kb，且转录方向相反。SAA4位于SAA2下游9 kb处，转录方向相同。SAA3位于SAA4下游110 kb处，其相对方向无法确定。所有已知与SAA位于相同人类和小鼠同线连锁群的基因都定位在该重叠群内。采用间期荧光原位杂交技术来确定该区域相对于着丝粒的方向：着丝粒 - LDHC - LDHA - SAA1 - SAA2 - SAA4 - SAA3 - TP H - D11S18 - KCNC1 - MYOD1 - 端粒。
