Monaghan G, Clarke D J, Povey S, See C G, Boxer M, Burchell B
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital Medical School, Scotland, United Kingdom.
Genomics. 1994 Sep 15;23(2):496-9. doi: 10.1006/geno.1994.1531.
Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.
此前我们已将编码人胆汁酸UDP-葡萄糖醛酸基转移酶（UGT2B4）的基因定位到4号染色体上。在此，我们报告利用聚合酶链反应（PCR）和一组人/啮齿动物体细胞杂交细胞系，将另外两个UGT2B基因定位到4号染色体上。分离出一个包含UGT2B4、UGT2B9和UGT2B15基因的酵母人工染色体重叠群，脉冲场凝胶电泳和PCR显示，人UGT2B基因亚家族的几个成员聚集在酵母人工染色体重叠群的一个195kb区域内。这些数据允许对基因进行初步排序，即UGT2B9-UGT2B4-UGT2B15。使用酵母人工染色体DNA进行的荧光原位杂交分析，将该基因簇定位到4号染色体的4q13区域。
