Aiba S, Nakagawa S, Ozawa H, Miyake K, Yagita H, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sandai, Japan.
J Invest Dermatol. 1993 Feb;100(2):143-7. doi: 10.1111/1523-1747.ep12462783.
After hapten application, epidermal Langerhans cells migrate into the regional lymph nodes through dermal lymphatics. Recently, we have demonstrated that some of them take the phenotypic and functional characteristics similar to those of in vitro cultured Langerhans cells, before disappearing from the epidermis. To analyze the mechanisms underlying the migration of Langerhans cells, we studied the expression of several adhesion molecules on freshly isolated LC and cultured LC. Pgp-1 (CD44), intercellular adhesion molecule 1, and alpha 4 integrin were strongly expressed on cultured Langerhans cells. Among them, only alpha 4 integrin was strongly up-regulated by cultured Langerhans cells, because its expression by freshly isolated Langerhans cells was very weak. This up-regulation of alpha 4 integrin was also observed on in vivo activated Langerhans cells in the epidermis and draining lymph nodes after hapten application. These data suggest a possible role played by VLA-4 in the migration of Langerhans cells from the epidermis into the regional lymph nodes after hapten application.
在应用半抗原后，表皮朗格汉斯细胞通过真皮淋巴管迁移至局部淋巴结。最近，我们已证明，其中一些细胞在从表皮消失之前，呈现出与体外培养的朗格汉斯细胞相似的表型和功能特征。为分析朗格汉斯细胞迁移的潜在机制，我们研究了新鲜分离的朗格汉斯细胞和培养的朗格汉斯细胞上几种黏附分子的表达情况。Pgp-1（CD44）、细胞间黏附分子1和α4整合素在培养的朗格汉斯细胞上强烈表达。其中，只有α4整合素在培养的朗格汉斯细胞上强烈上调，因为新鲜分离的朗格汉斯细胞对其表达非常弱。在应用半抗原后，在表皮和引流淋巴结中体内活化的朗格汉斯细胞上也观察到α4整合素的这种上调。这些数据表明VLA-4在应用半抗原后朗格汉斯细胞从表皮迁移至局部淋巴结的过程中可能发挥作用。
