Cohen S B, Katsikis P D, Feldmann M, Londei M
Kennedy Institute of Rheumatology, Sunley Division, Hammersmith, London.
Immunology. 1994 Nov;83(3):329-32.
Interleukin-10 (IL-10) has various immunomodulatory actions depending on the target cell type. Some of these effects have been shown to be owing to its ability to down-regulate surface expression of markers, for example HLA-DR on macrophages and CD25 (IL-2 receptor alpha chain) on B cells. In this report we show that preincubation of IL-10 for 24 hr up-regulates expression of the activation marker CD25, but not HLA-DR on cloned T cells of various phenotypes such as CD4+, CD8+, CD4- CD8- alpha beta and gamma delta T-cell receptor (TCR)-expressing cells. This up-regulation of CD25 was accompanied by an increase in the T cells IL-2-dependent proliferative response in 63% of the CD4+ clones and 100% of the CD8+, CD4-, CD8- alpha beta and gamma delta TCR+ clones analysed. IL-10 was also shown to be at least partly responsible for the up-regulation of CD25 on mitogen-activated peripheral blood mononuclear cells, suggesting that IL-10 has this CD25 modulatory effect within a more physiological environment. Our data suggest that IL-10 can have a multitude of effects on human T cells, and should not be considered exclusively as an immunoinhibitory cytokine.
白细胞介素-10（IL-10）根据靶细胞类型具有多种免疫调节作用。其中一些作用已被证明归因于其下调标志物表面表达的能力，例如巨噬细胞上的HLA-DR和B细胞上的CD25（IL-2受体α链）。在本报告中，我们表明，用IL-10预孵育24小时可上调各种表型的克隆T细胞（如CD4 +、CD8 +、CD4 - CD8 - αβ和γδ T细胞受体（TCR）表达细胞）上激活标志物CD25的表达，但不会上调HLA-DR的表达。在分析的63%的CD4 +克隆和100%的CD8 +、CD4 -、CD8 - αβ和γδ TCR +克隆中，CD25的这种上调伴随着T细胞IL-2依赖性增殖反应的增加。IL-10还被证明至少部分负责有丝分裂原激活的外周血单个核细胞上CD25的上调，这表明IL-10在更生理的环境中具有这种CD25调节作用。我们的数据表明，IL-10可对人T细胞产生多种影响，不应仅被视为一种免疫抑制细胞因子。
