Corell A, Morales P, Martínez-Laso J, Martín-Villa J, Varela P, Paz-Artal E, Allende L M, Rodríguez C, Arnaiz-Villena A
Department of Immunology, 12th of October University Hospital, Complutense University of Madrid, Spain.
Hum Immunol. 1994 Sep;41(1):52-5. doi: 10.1016/0198-8859(94)90084-1.
Six different ape MHC-G DNA sequences (four in humans: HLA-G01011, HLA-GII, HLA-G0103, and HLA-GIV; one in chimpanzees: Patr-GI; and one in gorillas: Gogo-G1) have been obtained. Only synonymous or conservative ("Thr"-to-"Ser") substitutions are allowed between the four human alleles. One allele of MHC-G exon-2 sequences has been found both in gorilla (Gorilla gorilla) and chimpanzee (Pan troglodytes). The Patr-GI DNA sequence shows two nonsynonymous substitutions when compared with the human HLA-G01011 sequence: "CGG"-to-"TGG" ("Arg"-to-"Trp") at codon 35 and "ATG"-to-"ATA" ("Met"-to-"Ile") at codon 76. One nonsynonymous "GAG"-to-"GGG" ("Glu"-to-"Gly") substitution is observed in the Gogo-GI exon-2 DNA sequence, when compared with the human 01011 allele. None of these three different substitutions have been observed in humans and are, thus, considered species specific. Also, evidence is provided that the human HLA-GII and G0103 may have been originated after human speciation. Finally, phylogenetic relationships among the six MHC-G alleles, tamarins G-"like" alleles, and other human class I genes (both "classical" and "nonclassic") are discussed.
已获得六种不同的猿类MHC - G DNA序列（人类四种：HLA - G01011、HLA - GII、HLA - G0103和HLA - GIV；黑猩猩一种：Patr - GI；大猩猩一种：Gogo - G1）。在这四个人类等位基因之间只允许同义或保守的（“苏氨酸”到“丝氨酸”）替换。在大猩猩（Gorilla gorilla）和黑猩猩（Pan troglodytes）中都发现了一个MHC - G外显子2序列的等位基因。与人类HLA - G01011序列相比，Patr - GI DNA序列显示出两个非同义替换：密码子35处的“CGG”到“TGG”（“精氨酸”到“色氨酸”）以及密码子76处的“ATG”到“ATA”（“甲硫氨酸”到“异亮氨酸”）。与人类01011等位基因相比，在Gogo - GI外显子2 DNA序列中观察到一个非同义的“GAG”到“GGG”（“谷氨酸”到“甘氨酸”）替换。这三种不同的替换在人类中均未观察到，因此被认为是物种特异性的。此外，有证据表明人类HLA - GII和G0103可能在人类物种形成之后才出现。最后，讨论了六种MHC - G等位基因、绢毛猴G - “类”等位基因以及其他人类I类基因（包括“经典”和“非经典”）之间的系统发育关系。
