Kozlosky C J, Maraskovsky E, McGrew J T, VandenBos T, Teepe M, Lyman S D, Srinivasan S, Fletcher F A, Gayle R B, Cerretti D P
Department of Gene Expression, Immunex Research and Development Corporation, Seattle, Washington 98101.
Oncogene. 1995 Jan 19;10(2):299-306.
Hek is a member of the eph subfamily of receptor tyrosine kinases whose members include elk, hek2, sek, eph and eck among others. Using a soluble form of hek consisting of the extracellular region of the receptor fused to the Fc domain of human IgG1 and an expression cloning strategy, we have isolated two different but related cDNAs from the human T-lymphoma line HSB-2 that encode ligands for hek. The cDNAs encode proteins of 238 and 201 amino acids (44% amino acid identity) that are anchored to the membrane by glycosylphosphatidylinositol (GPI)-linkage. The proteins encoded by these cDNAs are bound by hek with affinity constants of 2 x 10(8) M-1. These proteins also bind the elk tyrosine kinase receptor. These cDNAs are related to other cDNAs that we have recently isolated from a human placental library that encode ligands for both hek and elk and define an emerging family of ligands for eph-related kinases (LERKs).
Hek是受体酪氨酸激酶eph亚家族的成员，该家族成员还包括elk、hek2、sek、eph和eck等。利用由与人类IgG1的Fc结构域融合的受体细胞外区域组成的可溶性Hek形式以及表达克隆策略，我们从人T淋巴瘤细胞系HSB-2中分离出了两个不同但相关的cDNA，它们编码Hek的配体。这些cDNA编码238和201个氨基酸的蛋白质（氨基酸同一性为44%），它们通过糖基磷脂酰肌醇（GPI）连接锚定在膜上。这些cDNA编码的蛋白质与Hek结合，亲和常数为2×10⁸ M⁻¹。这些蛋白质也结合elk酪氨酸激酶受体。这些cDNA与我们最近从人胎盘文库中分离出的其他cDNA相关，那些cDNA编码Hek和elk的配体，并定义了一个新出现的eph相关激酶（LERKs）配体家族。
