一种改良的液相阻断酶联免疫吸附测定法用于评估泰国O型口蹄疫病毒的抗原变异情况。

A modified liquid phase (LP) blocking ELISA used to assess type O foot-and-mouth disease virus antigenic variation in Thailand.

作者信息

Lunt R A, Linchongsubongkoch W, Gleeson L J

机构信息

CSIRO Australian Animal Health Laboratory, Geelong, Vic., Australia.

出版信息

Vet Microbiol. 1994 Sep;42(1):79-90. doi: 10.1016/0378-1135(94)90079-5.

DOI:10.1016/0378-1135(94)90079-5

PMID:7839587

Abstract

A selection of type O foot-and-mouth disease (FMD) viruses isolated in Thailand between 1986 and 1989 were compared to the reference viruses O1 Thailand 1960 (O BKK/60) and O Nakorn Pathom 1965 (O NPT/65) using a liquid-phase blocking ELISA (LP ELISA) to derive serum titres and associated r values. Interpolation techniques were used to increase the precision for estimation of r values through a more accurate estimation of serum titres at predicted equivalent levels of antigen input. Mean r values were 0.45 (for 56 field viruses) relative to O BKK/60 reference virus and 0.56 (for 51 field viruses) relative to O NPT/65. While only two viruses showed considerable difference (r < 0.20) to a reference virus (O BKK/60), 41% and 31% gave r values less than 0.4 for O BKK/60 and O NPT/65 respectively. This indicated antigenic differences between reference and field viruses which may result in a reduction in vaccine efficacy.

摘要

采用液相阻断酶联免疫吸附试验（LP ELISA），对1986年至1989年期间在泰国分离出的一组O型口蹄疫（FMD）病毒与参考病毒O1泰国1960（O BKK/60）和O那空拍侬1965（O NPT/65）进行比较，以得出血清滴度和相关的r值。通过更准确地估计预测等效抗原输入水平下的血清滴度，采用插值技术提高r值估计的精度。相对于O BKK/60参考病毒，56株野毒株的平均r值为0.45，相对于O NPT/65，51株野毒株的平均r值为0.56。虽然只有两种病毒与参考病毒（O BKK/60）有显著差异（r < 0.20），但分别有41%和31%的病毒相对于O BKK/60和O NPT/65的r值小于0.4。这表明参考病毒与野毒株之间存在抗原差异，可能导致疫苗效力降低。