Veningerová M, Prachar V, Uhnák J, Kovacicová J
Institute of Preventive and Clinical Medicine, Bratislava, Slovakia.
Z Lebensm Unters Forsch. 1994 Oct;199(4):317-21. doi: 10.1007/BF01193319.
A method has been developed for the isolation, separation and determination of chlorinated phenols in total diet. The isolation takes place following acid hydrolysis of the conjugates and is performed by simultaneous extraction and distillation with water vapour and toluene. The isolated chlorophenols are removed by clean-up on a Florisil column using a mixture of 15% dichloromethane in toluene for elution. The separation and determination is carried out by capillary gas chromatography with electron capture detection. The recovery of the method ranged between 70.1 +/- 5 and 96.8 +/- 4.9% for the individual chlorophenols. The determination limits were 0.5 to 1.0 microgram.kg-1. The method was applied in a study aimed at estimating the chlorophenol burden in two population groups (children up to the age of 6 years and students up to the age of 18 years). In two successive years, 80 total diet samples were analysed. Average contents of the individual chlorophenols were from 0.7 to 33.5 micrograms.kg-1 and were higher for the dichlorophenol isomers. The calculated average daily intake ranged from 0.04 to 1.69 micrograms.kg-1 body weight for the individual phenols.
已开发出一种用于分离、提纯和测定总膳食中氯酚的方法。在对结合物进行酸水解后进行分离，通过水蒸气和甲苯同时萃取和蒸馏来完成。分离出的氯酚通过在弗罗里硅土柱上净化，用15%二氯甲烷的甲苯混合物洗脱。采用带有电子捕获检测的毛细管气相色谱法进行分离和测定。该方法对各氯酚的回收率在70.1±5%至96.8±4.9%之间。测定限为0.5至1.0微克·千克⁻¹。该方法应用于一项旨在估算两个人群组（6岁以下儿童和18岁以下学生）氯酚摄入量的研究。连续两年分析了80份总膳食样本。各氯酚的平均含量为0.7至33.5微克·千克⁻¹，二氯酚异构体的含量更高。计算得出的各酚类物质的平均每日摄入量为0.04至1.69微克·千克⁻¹体重。
