Charles L A, McGlasson D L, Hawksworth B A, Ashcraft J H, Ortel T L
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Blood Coagul Fibrinolysis. 1994 Aug;5(4):601-4.
Staclot LA is a hexagonal (II) phase phospholipid clotting assay used to confirm the presence of lupus anticoagulants (LA). However, there have been complaints that the procedure contains several incubation steps requiring 15 min of operator time. The authors were able to shorten this procedure to a single 5 min incubation without affecting assay sensitivity. Both procedures were performed on 45 known lupus anticoagulant positive specimens, 25 normal donors, eleven plasmas from patients with known factor VIII or factor V inhibitors and ten other specimens submitted for lupus anticoagulant or anticardiolipin antibody testing but without complete testing to confirm the presence of LA prior to testing with Staclot LA. Excellent agreement was observed between the two procedures with concurrence in 87 of 91 specimens (95.6%). Each method detected 39 of 45 LA positive specimens giving a sensitivity of 86.7%. This modification shortens technologist time by two-thirds without compromising assay sensitivity, which will allow for automation on commonly used coagulation analysers.
Staclot LA是一种用于确认狼疮抗凝物（LA）存在的六方（II）相磷脂凝血试验。然而，有人抱怨该方法包含几个孵育步骤，需要操作人员15分钟的时间。作者能够将该方法缩短为单次5分钟孵育，而不影响检测灵敏度。两种方法都对45份已知狼疮抗凝物阳性标本、25名正常供者、11份来自已知因子VIII或因子V抑制剂患者的血浆以及10份提交用于狼疮抗凝物或抗心磷脂抗体检测但在使用Staclot LA检测前未完成确认LA存在的完整检测的其他标本进行了检测。两种方法之间观察到极好的一致性，91份标本中有87份一致（95.6%）。每种方法检测到45份LA阳性标本中的39份，灵敏度为86.7%。这种改进将技术人员的时间缩短了三分之二，同时不影响检测灵敏度，这将允许在常用的凝血分析仪上实现自动化。
