Zheng Y J, Wang R H, Lin Y Z, Daniel T M
Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xian, Shaanxi, China.
Tuber Lung Dis. 1994 Oct;75(5):366-70. doi: 10.1016/0962-8479(94)90083-3.
Department of Paediatrics in a University Hospital.
To evaluate ABC-ELISA as serodiagnostic tests for tuberculosis in children.
An avidin-biotin-peroxidase conjugated anti-human immunoglobulin (ABC) enzyme-linked immunosorbent assay (ELISA) was established to measure serum immunoglobulin G (IgG) antibodies to polymerized old tuberculin (OT), tuberculin purified protein derivative (PPD) and the 30,000 dalton (30 kDa) native antigen of Mycobacterium tuberculosis, in 122 tuberculosis patients and 187 healthy children.
In those patients who had received less than 3 months of chemotherapy the sensitivities of the tests based on OT, PPD and the 30 kDa antigen were 40.3%, 50.0% and 36.1% respectively; the positive predictive values were 92.6%, 94.7% and 93.8% respectively and the negative predictive values were 58.6%, 62.9% and 57.2% respectively.
The ABC-ELISA may be useful for the diagnosis of pulmonary and extrapulmonary tuberculosis in groups of children with a high prior probability of tuberculosis. Antibodies to the 30 kDa antigen persisted longer than did those to OT and PPD.
大学医院儿科。
评估ABC-ELISA作为儿童结核病血清学诊断试验的价值。
建立了一种抗生物素蛋白-生物素-过氧化物酶结合抗人免疫球蛋白（ABC）酶联免疫吸附测定法（ELISA），用于检测122例结核病患者和187例健康儿童血清中针对聚合旧结核菌素（OT）、结核菌素纯蛋白衍生物（PPD）和结核分枝杆菌30000道尔顿（30 kDa）天然抗原的免疫球蛋白G（IgG）抗体。
在接受化疗少于3个月的患者中，基于OT、PPD和30 kDa抗原的试验敏感性分别为40.3%、50.0%和36.1%；阳性预测值分别为92.6%、94.7%和93.8%，阴性预测值分别为58.6%、62.9%和57.2%。
ABC-ELISA可能有助于对结核病患病可能性较高的儿童群体进行肺和肺外结核病的诊断。针对30 kDa抗原的抗体比针对OT和PPD的抗体持续时间更长。
