Skrypka I, Somerville R L
Department of Biochemistry, Purdue University, West Lafayette, IN 47907-1153.
DNA Seq. 1994;4(6):355-60. doi: 10.3109/10425179409010185.
The sequence of the Salmonella typhimurium trpR gene and flanking DNA was determined on both strands. The DNA sequence predicts a polypeptide product of 108 amino acids with a molecular weight of 12,274 daltons. The TrpR protein of S. typhimurium differs by three amino acid residues from that of E. coli. The promoter/operator region of trpR is completely conserved between E. coli and S. typhimurium. The nucleotide sequence of the trpR sector of the S. typhimurium genome was 87.4% identical to the corresponding region of the E. coli genome. Within the protein coding segments of the two organisms, 94.4% of the amino acid residues were identical. In S. typhimurium, as in E. coli, there is a Palindromic Unit element (PU) between the translation termination triplet of trpR and that of a divergently oriented unidentified reading frame (URF-143). However, the PU segment of S. typhimurium is 85 nucleotides shorter than its E. coli counterpart.
对鼠伤寒沙门氏菌trpR基因及其侧翼DNA的两条链进行了测序。DNA序列预测其多肽产物为108个氨基酸，分子量为12274道尔顿。鼠伤寒沙门氏菌的TrpR蛋白与大肠杆菌的TrpR蛋白有三个氨基酸残基的差异。trpR的启动子/操纵子区域在大肠杆菌和鼠伤寒沙门氏菌之间完全保守。鼠伤寒沙门氏菌基因组trpR区段的核苷酸序列与大肠杆菌基因组相应区域的一致性为87.4%。在这两种生物的蛋白质编码区段内，94.4%的氨基酸残基是相同的。与大肠杆菌一样，在鼠伤寒沙门氏菌中，trpR的翻译终止三联体与一个反向排列的未鉴定阅读框（URF-143）的翻译终止三联体之间存在一个回文单元元件（PU）。然而，鼠伤寒沙门氏菌的PU区段比其大肠杆菌对应区段短85个核苷酸。
