Law S W, Conneely O M, O'Malley B W
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Gene Expr. 1994;4(1-2):77-84.
We have cloned a novel member (mTR2R1) of the nuclear receptor superfamily of transcription factors from a mouse brain cDNA library. The cDNA sequence predicts a protein primary structure of 629 amino acids with a calculated molecular weight of 68.8 kDa. The amino acid sequence of the protein contains three regions of consensus sequence that are conserved throughout the nuclear receptor superfamily. One of these regions encodes a type II zinc finger DNA binding domain that is characteristic of this family of transcription factors. Comparison of the amino acid sequence of mTR2R1 with other nuclear receptors indicates that it is most closely related to the orphan receptor, hTR2, and suggests that these proteins constitute a novel subfamily within the nuclear receptor superfamily. mTR2R1 is encoded by two mRNAs that show different but overlapping spatial patterns of expression in the adult mouse. The identification of a subfamily of TR2 receptors together with the existence of variant mRNAs for these receptors prompts a close examination of the tissue-specific regulatory role of this subfamily of nuclear receptors.
我们从小鼠脑cDNA文库中克隆了转录因子核受体超家族的一个新成员（mTR2R1）。cDNA序列预测该蛋白一级结构由629个氨基酸组成，计算分子量为68.8 kDa。该蛋白的氨基酸序列包含三个在整个核受体超家族中保守的共有序列区域。其中一个区域编码II型锌指DNA结合结构域，这是该转录因子家族的特征。mTR2R1的氨基酸序列与其他核受体的比较表明，它与孤儿受体hTR2关系最为密切，并提示这些蛋白在核受体超家族中构成一个新的亚家族。mTR2R1由两种mRNA编码，它们在成年小鼠中显示出不同但重叠的表达空间模式。TR2受体亚家族的鉴定以及这些受体存在变异mRNA，促使人们仔细研究该核受体亚家族的组织特异性调节作用。
