Seppo A, Penttilä L, Leppänen A, Maaheimo H, Niemelä R, Helin J, Wieruszeski J M, Renkonen O
Institute of Biotechnology, University of Helsinki, Finland.
Glycoconj J. 1994 Jun;11(3):217-25. doi: 10.1007/BF00731221.
alpha 1,3-Galactosylation of radiolabelled bi-antennary acceptors Gal beta 1-4GlcNAc beta 1-3(Gal beta 1-4GlcNAc beta 1-6)Gal-R (R = 1-OH, beta 1-4GlcNAc or beta 1-4Glc) with bovine thymus alpha 1,3-galactosyltransferase was studied. At all stages of the reactions the three acceptors reacted faster at the 1-->6 linked arm than at the 1-->3 linked branch. Hence, in addition to the doubly alpha 1,3-galactosylated products, practically pure Gal beta 1-4GlcNAc beta 1-3(Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-6)Gal-R could be obtained from the three acceptors in reactions that had proceeded to near completion. The isomeric mono-alpha 1,3-galactosylated products were identified by using exoglycosidases to remove the branches unprotected by alpha 1,3-galactoses and by subsequently identifying the resulting linear glycans chromatographically.
研究了用牛胸腺α1,3 - 半乳糖基转移酶对放射性标记的双天线受体Galβ1 - 4GlcNAcβ1 - 3(Galβ1 - 4GlcNAcβ1 - 6)Gal - R（R = 1 - OH、β1 - 4GlcNAc或β1 - 4Glc）进行α1,3 - 半乳糖基化。在反应的所有阶段，这三种受体在1→6连接臂处的反应速度比在1→3连接分支处更快。因此，除了双α1,3 - 半乳糖基化产物外，在接近完全反应的反应中，从这三种受体中实际上可以获得几乎纯的Galβ1 - 4GlcNAcβ1 - 3(Galα1 - 3Galβ1 - 4GlcNAcβ1 - 6)Gal - R。通过使用外切糖苷酶去除未被α1,3 - 半乳糖保护的分支，并随后通过色谱法鉴定所得的线性聚糖，来鉴定异构体单α1,3 - 半乳糖基化产物。
