O'Neill W A, Cooke R P
Department of Medical Microbiology, District General Hospital, East Sussex, England, UK.
Br J Biomed Sci. 1994 Mar;51(1):1-4.
Eighty five beta-haemolytic Lancefield group A(23), C(22), and G(40) streptococci were tested for sulphonamide sensitivity, ability to degrade beta-D glucuronide (groups C and G) and bacitracin sensitivity (group A). Identification of isolates was initially confirmed by the API 20 STREP system. Zones of beta-haemolysis were too variable for correct Streptococcus milleri identification in 10% of cases. However, only group C and G S. milleri were both sulphonamide-resistant and beta-D glucuronide-negative. Two group A S. milleri strains could have been mis-identified as Streptococcus pyogenes if combined sulphonamide and bacitracin resistance had not been noted. In a busy diagnostic laboratory, screening of beta-haemolytic group A, C, and G streptococci for sulphonamide sensitivity is recommended. Sulphonamide-resistant group A S. pyogenes can be distinguished from S. milleri by bacitracin sensitivity. S. milleri can be rapidly and cheaply differentiated from other sulphonamide-resistant group C and G streptococci by a simple 4 h commercial beta-D glucuronide assay.
对85株β-溶血性A群（23株）、C群（22株）和G群（40株）链球菌进行了磺胺类药物敏感性、降解β-D-葡萄糖醛酸的能力（C群和G群）以及杆菌肽敏感性（A群）检测。分离株的鉴定最初通过API 20 STREP系统进行确认。在10%的病例中，β-溶血环变化太大，无法正确鉴定米勒链球菌。然而，只有C群和G群米勒链球菌对磺胺类药物耐药且β-D-葡萄糖醛酸阴性。如果没有注意到联合磺胺类药物和杆菌肽耐药性，两株A群米勒链球菌菌株可能会被误鉴定为化脓性链球菌。在繁忙的诊断实验室中，建议对β-溶血性A群、C群和G群链球菌进行磺胺类药物敏感性筛查。对磺胺类药物耐药的A群化脓性链球菌可通过杆菌肽敏感性与米勒链球菌区分开来。通过简单的4小时商业β-D-葡萄糖醛酸检测，可快速且低成本地将米勒链球菌与其他对磺胺类药物耐药的C群和G群链球菌区分开来。
