Haraguchi Y, Baba M, Takao S, Yoshinaka H, Hase S, Aikou T
First Department of Surgery, Kagoshima University School of Medicine, Japan.
Cancer. 1995 Feb 15;75(4):914-9. doi: 10.1002/1097-0142(19950215)75:4<914::aid-cncr2820750404>3.0.co;2-6.
There are few studies of flow cytometric analysis for DNA heterogeneity of patients with superficial carcinoma of the esophagus limited to the epithelium or superficially invading the lamina propria or submucosa.
Flow cytometric analysis of cellular DNA content was performed on superficial carcinomas of the esophagus using paraffin embedded blocks of the surgically resected specimens from 56 patients. To evaluate the intratumoral DNA heterogeneity, a total of 141 samples of the 56 tumors were analyzed, depending upon the tumor size.
One or two of the samples was available from 18 of 19 patients with tumors 2 cm or less in greatest dimension, whereas more than three of the samples were available from 22 of 37 patients with tumors 2.1 cm or greater in dimension (P < 0.003). Of 56 tumors, 40 (71.4%) exhibited DNA aneuploidy; DNA heterogeneity was found in 26 tumors (46.4%). The remaining 16 tumors exhibited DNA diploidy. Two of the five tumors that were limited to the epithelium had DNA heterogeneity. The mean dimension of the tumors with DNA heterogeneity was significantly greater (5.8 +/- 2.8 cm) than those exhibiting DNA diploidy (2.3 +/- 1.1 cm) and DNA aneuploidy without heterogeneity (2.9 +/- 2.4 cm). Recurrences after esophagectomy were detected in 6 of the 56 patients; the DNA ploidy pattern of these six patients exhibited DNA heterogeneity.
The incidence of DNA heterogeneity increases as tumor size increases and is associated with an increased risk of tumor recurrence after esophagectomy in patients with superficial carcinoma of the esophagus.
针对局限于上皮层或浅表侵犯固有层或黏膜下层的食管表浅癌患者的DNA异质性进行流式细胞术分析的研究较少。
对56例患者手术切除标本的石蜡包埋块进行食管表浅癌的细胞DNA含量流式细胞术分析。为评估肿瘤内DNA异质性，根据肿瘤大小，对56个肿瘤共141个样本进行了分析。
最大径2cm或更小的19例患者中，18例可获得1或2个样本，而最大径2.1cm或更大的37例患者中，22例可获得3个以上样本（P<0.003）。56个肿瘤中，40个（71.4%）表现为DNA非整倍体；26个肿瘤（46.4%）存在DNA异质性。其余16个肿瘤表现为DNA二倍体。局限于上皮层的5个肿瘤中有2个存在DNA异质性。存在DNA异质性的肿瘤平均大小（5.8±2.8cm）显著大于表现为DNA二倍体的肿瘤（2.3±1.1cm）及存在DNA非整倍体但无异质性的肿瘤（2.9±2.4cm）。56例患者中有6例在食管切除术后出现复发；这6例患者的DNA倍体模式表现为DNA异质性。
食管表浅癌患者中，DNA异质性的发生率随肿瘤大小增加而升高，且与食管切除术后肿瘤复发风险增加相关。
