Ito M, Watanabe M, Kamiya H, Sakurai M
Department of Pediatrics, Mie University School of Medicine, Japan.
Cell Immunol. 1995 Jan;160(1):8-13. doi: 10.1016/0008-8749(95)80003-2.
The expression of adhesion molecules (LFA-1, ICAM-1, CD29) on T cells activated with cytomegalovirus (CMV) antigen was investigated by three-color flow cytometry analysis. Peripheral blood mononuclear cells (PBMC) from CMV-seropositive adults were cultured with CMV or control antigen for 6 days. After culture, the expression of LFA-1, ICAM-1, and CD29 on T cells in subpopulations defined as CD45RO+ or CD45RO- was analyzed. The population of cells that expressed LFA-1 at high levels (LFA-1high) among CD4+C-D45RO+ cells increased when cultured with CMV antigen compared to control antigen. The population of cells that expressed LFA-1high cells among CD8+C-D45RO+ cells was down-regulated by culture for 6 days; however, the population of LFA-1high cells among CD8+CD45RO+ cells cultured with CMV antigen was higher than when cultured with control antigen. The population of LFA-1high cells among CD4+CD45RO- and CD8+CD45RO- cells did not change after culture with CMV antigen. The intensity of CD29 and expression of ICAM-1 also increased on both CD4+CD45RO+ and CD8+CD45RO+ cells after culture with the CMV antigen. Up-regulation of adhesion molecules occurred on activated T cells by culture with the CMV antigen. This change was observed mainly on CD45RO+ T memory cells. The results suggested that these changes of adhesion molecules on activated T cells with CMV may contribute to some immune reaction or inflammatory change.
采用三色流式细胞术分析，研究了用巨细胞病毒（CMV）抗原激活的T细胞上黏附分子（淋巴细胞功能相关抗原-1、细胞间黏附分子-1、CD29）的表达情况。将来自CMV血清阳性成年人的外周血单个核细胞（PBMC）与CMV或对照抗原培养6天。培养后，分析定义为CD45RO+或CD45RO-的亚群中T细胞上淋巴细胞功能相关抗原-1、细胞间黏附分子-1和CD29的表达。与对照抗原相比，用CMV抗原培养时，CD4+C-D45RO+细胞中高水平表达淋巴细胞功能相关抗原-1（淋巴细胞功能相关抗原-1高表达）的细胞群体增加。CD8+C-D45RO+细胞中淋巴细胞功能相关抗原-1高表达细胞群体经6天培养后下调；然而，用CMV抗原培养的CD8+CD45RO+细胞中淋巴细胞功能相关抗原-1高表达细胞群体高于用对照抗原培养时。用CMV抗原培养后，CD4+CD45RO-和CD8+CD45RO-细胞中淋巴细胞功能相关抗原-1高表达细胞群体未发生变化。用CMV抗原培养后，CD4+CD45RO+和CD8+CD45RO+细胞上CD29的强度和细胞间黏附分子-1的表达也增加。用CMV抗原培养可使活化T细胞上的黏附分子上调。这种变化主要在CD45RO+T记忆细胞上观察到。结果表明，CMV活化T细胞上这些黏附分子的变化可能有助于某些免疫反应或炎症变化。
