Qi Q, Dang G D, Cai Q L
Third Teaching Hospital, Beijing Medical University.
Zhonghua Wai Ke Za Zhi. 1994 Mar;32(3):187-9.
The lumbar intervertebral discs (L3-6) were ablated through a transperitoneal approach in 12 adult dogs by using Nd: YAG laser (1.06 microns) with a 600 microns quartz fiber. The status of limbs motion and sphincter (bladder, bowel) was observed for evaluating the safety of laser irradiation. After irradiation, the animals were sacrificed at prescribed intervals of up to 40 weeks (2, 4, 8, 12 and 40 weeks after operation). The lumbar intervertebral discs were harvested and subjected to light microscopic observation. No dog had suffered from neurogenic dysfunction of limb motion and sphincter. Histological findings immediately after the irradiation showed the disc was vaporized and a cavity was made. After 2 and 4 weeks, fibrous tissues began to proliferate, but cartilaginous tissues replaced the fibrous tissues 12 weeks after the laser irradiation. No new bone formation was found within 40 weeks after operation. On the basis of this study and our previous cadaveric study, percutaneous laser disc decompression (PLDD) was applied in clinical practice in march of 1993. 10 patients underwent PLDD utilizing the same laser equipment. The average follow-up was 3 months. According to the Macnab's criteria, there was an excellent response in 7 patients and a good response in 3.
采用经腹膜途径，使用波长为1.06微米的Nd:YAG激光及600微米石英光纤，对12只成年犬的腰3至腰6椎间盘进行消融。观察肢体运动及括约肌（膀胱、肠道）状态以评估激光照射的安全性。照射后，在规定的最长40周间隔时间（术后2、4、8、12和40周）处死动物。取出腰段椎间盘进行光镜观察。所有犬均未出现肢体运动和括约肌神经源性功能障碍。照射后即刻的组织学检查发现椎间盘被汽化形成空洞。2周和4周后，纤维组织开始增生，但激光照射12周后软骨组织取代了纤维组织。术后40周内未发现新骨形成。基于本研究及我们之前的尸体研究，1993年3月将经皮激光椎间盘减压术（PLDD）应用于临床实践。10例患者使用相同的激光设备接受了PLDD治疗。平均随访3个月。根据Macnab标准，7例患者疗效极佳，3例患者疗效良好。
