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Environ Health Perspect. 1994 Sep;102 Suppl 3(Suppl 3):301-4. doi: 10.1289/ehp.94102s3301.
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Developing ICP-MS/MS for the detection and determination of synthetic DNA-protein crosslink models via phosphorus and sulfur detection.开发用于通过磷和硫检测来检测和测定合成DNA-蛋白质交联模型的电感耦合等离子体质谱串联质谱（ICP-MS/MS）。
Anal Bioanal Chem. 2015 Mar;407(9):2433-7. doi: 10.1007/s00216-015-8504-x. Epub 2015 Feb 5.

本文引用的文献

1
Phosphodiester bonds between polypeptides and chromosomal DNA.多肽与染色体DNA之间的磷酸二酯键。
J Mol Biol. 1983 Feb 25;164(2):213-35. doi: 10.1016/0022-2836(83)90076-1.
2
The formation, identification, and significance of DNA-protein cross-links in mammalian cells.哺乳动物细胞中DNA-蛋白质交联的形成、鉴定及其意义。
Br J Cancer Suppl. 1987 Jun;8:135-40.
3
Toxicity and carcinogenicity of nickel compounds.镍化合物的毒性与致癌性。
Crit Rev Toxicol. 1989;19(4):341-84. doi: 10.3109/10408448909029327.
4
Analysis of proteins cross-linked to DNA after treatment of cells with formaldehyde, chromate, and cis-diamminedichloroplatinum(II).
Mol Toxicol. 1989 Winter;2(1):11-26.
5
Chemical nature of DNA-protein cross-links produced in mammalian chromatin by hydrogen peroxide in the presence of iron or copper ions.在铁离子或铜离子存在的情况下，过氧化氢在哺乳动物染色质中产生的DNA-蛋白质交联物的化学性质。
Biochemistry. 1991 May 21;30(20):4873-9. doi: 10.1021/bi00234a006.
6
The role of oxidative processes in metal carcinogenesis.
Chem Res Toxicol. 1991 Nov-Dec;4(6):592-604. doi: 10.1021/tx00024a001.
7
Analysis of DNA-protein complexes induced by chemical carcinogens.
J Cell Biochem. 1990 Nov;44(3):127-35. doi: 10.1002/jcb.240440302.
8
Analysis of residual amino acid--DNA crosslinks induced in intact cells by nickel and chromium compounds.镍和铬化合物诱导完整细胞产生的残留氨基酸-DNA交联的分析。
Carcinogenesis. 1992 Oct;13(10):1763-8. doi: 10.1093/carcin/13.10.1763.

开发一种作为DNA-蛋白质交联简单、快速且灵敏指标的¹²⁵I后标记检测法。

Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.

作者信息

Zhuang Z, Costa M

机构信息

Nelson Institute of Environmental Medicine, New York University Medical Center, New York 10016.

出版信息

Environ Health Perspect. 1994 Sep;102 Suppl 3(Suppl 3):301-4. doi: 10.1289/ehp.94102s3301.

DOI:10.1289/ehp.94102s3301
PMID:7843121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1567398/
Abstract

A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamster ovary cells were exposed to various crosslinking agents, e.g., UV light, K2CrO4, or NiCl2. DNA was isolated by proteinase K/phenol/chloroform. The residual peptides cross-linked to DNA were radioiodinated with Na125I and chloramine T. After repeated precipitation with ethanol, the radioactivity was determined. The 125I method was compared with a 3[H]-tyrosine prelabeling method and found to be of similar sensitivity.

摘要

已开发出一种快速、简单且灵敏的¹²⁵I后标记技术，用于检测环境污染物和致癌物诱导的DNA-蛋白质交联。该方法基于将¹²⁵I特异性掺入与DNA相关的酪氨酸残基中。将培养的中国仓鼠卵巢细胞暴露于各种交联剂，如紫外线、重铬酸钾或氯化镍。通过蛋白酶K/苯酚/氯仿分离DNA。与DNA交联的残留肽用¹²⁵I化钠和氯胺T进行放射性碘化。用乙醇反复沉淀后，测定放射性。将¹²⁵I方法与³[H]-酪氨酸预标记方法进行比较，发现灵敏度相似。