Thiele D, Willems H, Köpf G, Krauss H
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen, Germany.
Eur J Epidemiol. 1993 Jul;9(4):419-25. doi: 10.1007/BF00157400.
Pulsed field gel electrophoresis was used for differentiation of Coxiella burnetii isolates derived from animals in Europe and one from Namibia. Previously published data on isolates from North America demonstrated four groups with distinct banding patterns. Fingerprints of prototype isolates as references confirmed four different cleavage patterns upon NotI restriction of total DNA as described. With isolates available at our institute five additional DNA banding patterns were obtained from European and one Namibian isolate after endonuclease restriction with NotI. To compare patterns from different electrophoretic runs more precisely an image analysis system was used.
脉冲场凝胶电泳用于区分来自欧洲动物的伯氏考克斯氏体分离株以及来自纳米比亚的一株分离株。先前发表的关于北美分离株的数据显示有四组具有不同条带模式。作为参考的原型分离株的指纹图谱证实，如所述，在用NotI对总DNA进行酶切后有四种不同的切割模式。利用我们研究所现有的分离株，在用NotI进行核酸内切酶酶切后，从欧洲和一株纳米比亚分离株中获得了另外五种DNA条带模式。为了更精确地比较不同电泳结果的模式，使用了图像分析系统。
