Chida D, Kume T, Mukouyama Y, Tabata S, Nomura N, Thomas M L, Watanabe T, Oishi M
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
FEBS Lett. 1995 Jan 30;358(3):233-9. doi: 10.1016/0014-5793(94)01432-z.
From our previous studies, several protein tyrosine phosphatases (PTPase) are implicated in the early events leading to in vitro differentiation of both mouse erythroleukemia (MEL) and embryonal carcinoma (F9) cells. Among the PTPases, recent experiments suggest that a new PTPase (RIP) plays a critical role in differentiation processes, particularly at their early stages. We isolated cDNA clones for RIP from a RNA preparation isolated from differentiating MEL cells, and determined the total 7932 bp base sequence for RIP cDNA. The cDNA codes for a putative 269.8 kDa (2450 amino acids) protein with a PTPase catalytic domain. We have demonstrated that the transcripts exist in multiple forms, and among mouse tissues they were found predominantly in kidney and, to a lesser extent, in lung, heart, brain and testis. The RIP gene was mapped between D5Mit90 and D5Mit25 on mouse chromosome 5.
根据我们之前的研究，几种蛋白酪氨酸磷酸酶（PTPase）参与了小鼠红白血病（MEL）细胞和胚胎癌细胞（F9）体外分化的早期过程。在这些PTPase中，最近的实验表明，一种新的PTPase（RIP）在分化过程中，特别是在其早期阶段，发挥着关键作用。我们从分化的MEL细胞分离的RNA制备物中分离出RIP的cDNA克隆，并确定了RIP cDNA的全长7932 bp碱基序列。该cDNA编码一种推定的269.8 kDa（2450个氨基酸）的蛋白质，具有PTPase催化结构域。我们已经证明，该转录本存在多种形式，在小鼠组织中，它们主要存在于肾脏中，在肺、心脏、大脑和睾丸中含量较少。RIP基因定位于小鼠5号染色体上的D5Mit90和D5Mit25之间。
