Piekos M W, Binor Z, Rawlins R G, Radwanska E
Department of Obstetrics and Gynecology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612-3864.
Fertil Steril. 1995 Feb;63(2):371-6. doi: 10.1016/s0015-0282(16)57371-3.
To investigate the effect of induced endogenous hyperprolactinemia on the luteinization process, as expressed by the shift in the P:E2 ratio after hCG injection in IVF cycles.
Serum PRL, E2, and P levels were measured in 49 IVF patients (leuprolide acetate and hMG protocol) on the day of hCG injection. Estradiol and P also were measured on the day after hCG. Serum P:E2 ratios were calculated for two groups of patients; group I (control): PRL < or = 20 ng/mL (conversion factor to SI unit, 1.00); group II (hyperprolactinemia): PRL > 20 ng/mL. Estradiol and P also were measured in follicular fluid (FF) and the gamete performance was compared between groups.
Data analysis showed no significant differences in the mean +/- SD serum peak E2 (pg/mL; conversion factor to SI unit, 3.671) between groups: group I, 1,769 +/- 843; group II, 2,333 +/- 1,194; the mean FF E2 (pg/mL) group I, 351 +/- 221; group II, 370 +/- 186; or the mean FF P (ng/mL; conversion factor to SI unit, 3.180) group I, 8,357 +/- 3,127; group II, 11,354 +/- 12,888. No significant differences were found between groups in the P:E2 ratios on days 1 or 2: group I, 78 +/- 48 and 209 +/- 137; group II, 70 +/- 47 and 224 +/- 197, respectively. The magnitude of the P shift also showed no significant difference between the two groups; the mean +/- SD shift in the P level was 2.9 +/- 2.2 for group I, and 4.3 +/- 5.1 for group II. The serum PRL level had no effect on the fertilization rate (60% for group I and 70% for group II) or on the pregnancy rate (17% for group I and 23% for group II).
These findings suggest that mild endogenous hyperprolactinemia induced by ovarian stimulation does not affect granulosa cell luteinization and gamete performance in humans.
通过体外受精（IVF）周期中注射人绒毛膜促性腺激素（hCG）后P:E2比值的变化来研究内源性高催乳素血症对黄体化过程的影响。
对49例接受IVF治疗的患者（醋酸亮丙瑞林和人绝经期促性腺激素方案）在注射hCG当天测定血清催乳素（PRL）、雌二醇（E2）和孕酮（P）水平。在注射hCG后的第二天也测定雌二醇和孕酮。计算两组患者的血清P:E2比值；第一组（对照组）：PRL≤20 ng/mL（换算为国际单位的转换因子为1.00）；第二组（高催乳素血症组）：PRL＞20 ng/mL。还测定了卵泡液（FF）中的雌二醇和孕酮，并比较了两组之间的配子性能。
数据分析显示两组之间血清E2峰值的平均值±标准差（pg/mL；换算为国际单位的转换因子为3.671）无显著差异：第一组为1769±843；第二组为2333±1194；第一组卵泡液中E2的平均水平（pg/mL）为351±221；第二组为370±186；或者第一组卵泡液中P的平均水平（ng/mL；换算为国际单位的转换因子为3.180）为8357±3127；第二组为11354±12888。两组在第1天或第2天的P:E2比值无显著差异：第一组分别为78±48和209±137；第二组分别为70±47和224±197。两组之间P值变化的幅度也无显著差异；第一组P水平的平均±标准差变化为2.9±2.2，第二组为4.3±5.1。血清PRL水平对受精率（第一组为60%，第二组为70%）或妊娠率（第一组为17%，第二组为23%）没有影响。
这些发现表明，卵巢刺激诱导的轻度内源性高催乳素血症不会影响人类颗粒细胞的黄体化和配子性能。
